Bovine Deoxyribonuclease I RNase/Protease Free LyophilizedIBODNASEPRFLY2500UN
Bovine Deoxyribonuclease I RNase/Protease Free Lyophilized from Innovative Research has been chromatographically purified to remove RNase and protease. This is a lyophilized powder with a concentration of 2,000 Kunitz units/dry weight.
This product is suitable for use in techniques requiring digestion of DNA in the recovery of intact RNA or where the integrity of structural proteins or enzymes must be maintained. Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, RNA polymerase synthesis of RNA probes and RT-PCR. It is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. This is highly stable due to the absence of protease. For long term storage after reconstitution, use water or any buffer pH 4.0 to 9.0 except phosphate; add 50% glycerol for storage as liquid at -20°C; avoid calcium chelators. Aliquot in single use containers; only freeze and thaw once; thawed aliquots are stable refrigerated at least several weeks.
- Source: Bovine Pancreas
- Purification: chromatographically purified
- Storage Conditions: 2-8°C
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These products are supplied for manufacturing, research and laboratory use only. Researchers and laboratory personnel intending to use any of these products for medical investigation on humans are solely responsible for such use and for compliance with the pertinent regulations of the United States Food and Drug Administration (US-FDA) and other regulations. We do not assume liability for damages resulting from the handling, use and/or disposal of these products, from their use in violation of patent or other rights or reliance upon this information.
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