Rat IgM Assay and ELISA Kits


Catalog No.: IRTIGMKT


Category: Assay and ELISA Kits

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Product Description


IgM is the first immunoglobulin produced in the immune response and is the third most abundant immunoglobulin in serum. IgM is a disulfide-linked 970kDa pentamer that activates complement and is responsible for red blood cell agglutination. Each monomer consists of two mu heavy chains and two kappa or lambda light chains.

This Rat Immunoglobulin M (IgM) antigen assay is intended for the quantitative determination of total rat IgM antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay measures IgM antigen in the 0.5 - 500 ng/ml range. If the unknown is thought to have high IgM levels, dilutions may be made in blocking buffer. A 1:1,000 to 1:10,000 dilution for normal rat plasma or serum is suggested for best results. Strip well format. Reagents for up to 96 tests.

Rat IgM will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-rat IgM antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat IgM. Color development is directly proportional to the concentration of total IgM in the samples.

Detection Target: IgM
Reactivity: Rat
Range: 0.5 - 500 ng/ml
Sensitivity: 0.25 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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