Mouse Peptides Assay and ELISA Kits

Mouse Hemopexin ELISA Kit

Catalog No.: IRKTAH1153


Category: Assay and ELISA Kits

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Product Description

Mouse Hemopexin ELISA Kit

In this assay the Hemopexin present in samples reacts with the anti-hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for
sample dilution.

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Mouse Hemopexin ELISA Kit

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