Human Kappa ELISA Kit
Human Kappa ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Kappa in Human Biological Samples. In this assay the Kappa present in samples reacts with the anti-Kappa antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Kappa antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Kappa. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Kappa in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Kappa in the test sample. The quantity of Kappa in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.