Human Interferon gamma ELISA Kit
Human Interferon gamma ELISA kit is designed for detection of human IFN- in plasma, serum, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IFN- in less than 5 hours. A polyclonal antibody specific for human IFN- has been pre-coated onto a 96-well microplate with removable strips. IFN- in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for IFN- , which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.