Human Factor X ELISA Kit
Factor X is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor Xa. Factor X is activated by Factor IXa in complex with Factor VIII, calcium and phospholipids during the intrinsic pathway and by Factor VIIa in complex with Tissue Factor, calcium and phospholipids during the extrinsic pathway of the coagulation cascade.
This human coagulation Factor X antigen assay is intended for the quantitative determination of total Factor X antigen in human plasma. The assay measures Factor X antigen in the 0.1 - 50 ng/ml range. If the unknown is thought to have high Factor X levels, dilutions may be made in blocking buffer. For normal plasma 1:2,000-1:8,000 dilutions is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human Factor X will bind to the capture antibody coated on the microtiter plate. Factor X and Xa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor X present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor X. Color development is proportional to the concentration of Factor X in the samples.
Detection Target: Factor X
Range: 0.1 - 50 ng/ml
Sensitivity: 0.060 ng/ml
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