Human PAI-1 Upa Complex ELISA Kit
Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration. Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with uPA and tPA.
This Human PAI-1/uPA complex assay is intended for the quantitative determination of the covalent complex of uPA and its inhibitor PAI-1 in human plasma, serum, urine, tissue extracts, or cell culture media. The assay measures PAI-1/uPA complex in the 0.1 - 100ng/ml range. If the unknown is thought to have high complex levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.
Human uPA in samples will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-human PAI-1 primary antibody binds to PAI-1/uPA complex captured on the plate. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve of PAI-1/uPA complex is prepared along with the samples to be measured. Color development is proportional to the concentration of PAI-1/uPA complex in the samples. Free uPA and PAI-1 will not be detected by this assay.
Detection Target: PAI-1-UPA complex
Range: 0.1 - 100 ng/ml
Sensitivity: 0.070 ng/ml
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