Lab-Grown Skin to Replace Animal Testing

Written by cps on August 15, 2017. Posted in Blog

L’Oreal’s proprietary EpiSkin mimics human epidermis and spares lab animals, could one day lead to synthesized organs.

When you hear the name L’Oreal, you probably don’t think of gelatinous blobs of synthesized skin growing in a petri dish. But according to a recent CNBC article, that’s exactly what the beauty conglomerate is up to. To combat the public outcry against animal testing, L’Oreal researchers have created EpiSkin, a lab-produced tissue on which they can test the efficacy of ingredients before they hit the market.

The French company has also managed to monetize the product by selling it to other manufacturers of cosmetic, pharmaceutical, chemical and household products. They’ve also teamed up with California-based Organovo to 3-D print human skin with hair follicles that they intend to use for future products. The only competitor in the fabricated skin space is MatTek’s EpiDerm. The company uses human skin cells retrieved from cosmetic surgeries and circumcisions to produce roughly two adult humans’ worth of skin each week. MatTek's next step is organizing multiple tissue models in a “cross-talk” system to determine the effects of superficial products on liver toxicity and absorption by the small intestine.

Original Source: https://www.healthcarepackaging.com/article/applications/healthcare/lab-grown-skin-replace-animal-testing

Original Date: June 1 2017

Original Author: Tim Hayes

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Tips on Buying Human Biologicals

Written by cps on August 10, 2017. Posted in Blog

Buying human biologicals for research purposes as well as having patent right to them has been accompanied by lots of ethical questions for as long as they have been used.

Human biological fluid research materials are also accompanied by another issue, who is the rightful owner of a particular human biological material? Issues like this make it necessary for buyers to have information on when purchasing so that they do not fall prey to inadequate information that compromises valuable research.

What do we mean when we are discussing human biological research materials? In section four of the Health Research Act it defines human biologics as parts of organs, cells, tissues or other components of any material sourced from a human being whether they are alive or dead.

Tip #1 – Understand the property rights that are common to the human biological you wish to purchase.

To know the right of ownership of any human biological materials (such as the human biological fluid research material) is essential because it will evade you from unnecessary legal injunctions. This is particularly important when using materials in research. The last thing that is needed when doing research is complications on the research materials that are used.

A standard reference to such issues is seen in the case of Doctor Fremont and Simon. Doctor Fremont applied for a patent right to Fremont’s DNA and got approved but there was no legal precision on ownership of the molecular pathology materials. For more information and details on this case take a look into “the taking of human biologicals”.

The point is this: before you apply for a patent or buy any human biological you should have adequate knowledge about the property right of such human biologicals. Just a hint here! For human biologicals such as human biological fluids materials, the legal position on it is that patents do not cover it when they are still in the human body, but when they are outside the human body, the patent holder can exercise an exclusive right to such materials.

Tip #1 – Understand the requirements of the particular human biological fluid research materials you want to purchase.

Human biological fluid research materials such as blood, serum and plasma have standard requirements for the buyer. First, the blood will need to be stored in a refrigerator. This is a standard requirement for you as a buyer. Blood storage refrigeration is unlike traditional domestic refrigeration equipment. The following refers to some specifications that are unique to a blood storage refrigerator.

  • Must have an all round heavier insulation, which enables it to hold the required temperature in any case of power failure. With such insulin, it should be able to maintain temperatures between +20 and +60
  • A blood storage refrigerator should have a cooling fan that enables it to distribute air in the cabinet evenly.
  • It should have a good temperature monitoring device, which should comprise of an external temperature display and an alarm system for abnormal temperature or in the event of power failure.

In our next installment on buying human biological we will continue to look more in depth at the requirements of the particular human biological fluid research materials.

Learn more about Innovative Research and our high quality research materials including human, plant, and animal biological, ELISA & Assay Kits, proteins, antibodies, cell cultures, blood, serum, plasma, and more. More information can be found at https://www.innov-research.com/ or by calling 248.896.0145.

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A team of researchers at Synthetic Genomics (SG) has unveiled a machine they call a digital-to-biological converter—it sends digitized information describing DNA, RNA or a protein to a device that prints out synthesized versions of the original material. The team has published a paper describing their creation in the journal Nature Biotechnology.

What if you could launch a machine aboard a rocket to Mars and then transmit information describing a life form, which the machine then "prints?" That is what the team at SG envisions. Or more practically, they envision sending the digitized DNA of a deadly virus from a remote outbreak zone to a research lab that uses the information to develop a vaccine. Once created, the same converter machine could then be used to print the material for creating the vaccine locally.

The device is actually a hodgepodge of smaller devices that contribute to the whole. One of the main pieces is the BioXP 3200—a synthetic DNA printer that is already marketed around the world to researchers who use it to easily create synthetic DNA samples. The rest of the pieces receive information and process it and deal with the printed materials—the head of the project at SG, for example, can send a message from his office to the machine and then walk over and collect a virus it has created. This is not a means for creating life, the researchers note, because viruses are not considered forms of life. Instead, it is described as a "digital to biological converter for on-demand production of biologics."

Researchers at SG have used the device to remotely synthesize viruses and claim they are on the cusp of doing the same with a so-called minimal cell, a major step toward remotely printing material for creating living organisms. But first, they have to fix what they describe as an unacceptable rate of mutations.

In their paper and announcement, the company highlights the positive uses for the machine, but notably avoid mentioning the negative—the ability to use the machine to produce a virus at a given location that could be released as a biological weapon.

Read more at: https://phys.org/news/2017-08-synthetic-genomics-unveils-digital-to-biological-digital.html#jCp Original Author: Bob Yirka Original Date: August 7, 2017

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Animal biologicals include whole blood, plasma, serum, hormones, body fluid, glands, and tissues. The objective of collecting the biologicals is to ensure that they are free of every form of contamination. The principles that determine the collection of high-quality animal biologicals are determined by the animal source, the collection process and the storage of the products.

The animals in question should be antibiotic and anti-parasitic-free. The feed and water given to the animals must be certified to be free of any form of contamination. The animals should be healthy in every ramification, without merely the absence of disease. Sick or diseased animals should not be used as sources of biologicals. Any antibiotics to be administered to any animal should be certified by a veterinarian and must be given at least 21 days before the biologicals are collected. Antibiotic contamination limits the use of the biologicals and skews results. Parasitic contamination affects the integrity of tissue samples.

The samples should be collected when the animals are least stressful, as stress affects the quality of the samples. Sometimes, the biologicals have to be collected from genetically modified and pregnant animals for specific studies of such diseases as endocrine disease, diabetes, cancer and Alzheimers. The samples should be collected in the least painful manner. The conditions for the collection process must be aseptic in line with regulations for research. These regulations guide the restraint of the animals, the disinfection of the collection site, and the use of proper equipment. Collection sites should be shaved, and disinfected with 70% alcohol before and after the collection of blood. New syringes should be used for each animal, and used syringes should be disposed immediately and appropriately. Biological products may be collected at abattoirs pre-slaughter or from donor animals on farms.

Blood is collected though a vacutainer and then transferred into vials, bottles or bags. It is more preferable to use a vacutainer instead of a syringe. To collect sera for serological analysis, no anti-coagulants are used. The blood sample should be made to stand at ambient temperatures for 1-2 hours. If the collection of whole blood is the goal, then the collecting receptacle would contain one of the following anti-coagulants: Sodium Heparin, Alsevers Solution, Sodium EDTA, or Sodium Citrate. The different anticoagulants have different properties and are suitable for different laboratory procedures. Whole blood samples should be transported to the laboratory at 40C and not frozen.

Blood collection without fluid replacement is restricted to 10% of the total blood volume on a single occasion. Where blood collection would be regularly repeated, a canula may be used to reduce stress. At least 2.5ml and at most 10ml of blood should be collected per animal for an analysis.

Tissue harvest should be started immediately after euthanasia to prevent bacterial contamination through intestinal seepage. Organs should be observed in situ before tissue samples are removed. Tissues may be immediately fixed to prevent decay, autolysis and putrefaction, and thus preserve cells for examination. 10% buffered formalin is best for fixing tissues. The downside to preserving tissue with formalin is that it ruins the examination of the tissue for certain tests, such as microbial examination. Alternatively, tissue may be refrigerated at 40C for short periods or frozen at -100C for longer periods.

Learn more about Innovative Research and our high quality research materials including human, plant, and animal biological, ELISA & Assay Kits, proteins, antibodies, cell cultures, blood, serum, plasma, and more. More information can be found at https://www.innov-research.com/ or by calling 248.896.0145.

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Biologics: The Drugs Transforming Medicine

Written by cps on August 2, 2017. Posted in Blog

The Conversation
In a factory just outside San Francisco, there's an upright stainless steel vat the size of a small car, and it's got something swirling inside.

The vat is studded with gauges, hoses and pipes. Inside, it's hot just under 100 degrees Fahrenheit. Sugar and other nutrients are being pumped in because, inside this formidable container, there is life.

Scientists are growing cells in there. Those cells, in turn, are growing medicine. Every two weeks or so, the hot, soupy liquid inside gets strained and processed. The purified molecules that result will eventually be injected into patients with Stage IV cancer.

Researchers Look at Costs of New Tools to Fight Cancer

Drugs that are made this way inside living cells are called biologics. And they're taking medicine by storm. By 2016, biologics hadsurged to make up 25 percentof the total pharmaceutical market, bringing in 232 billion, with few signs their upward trend will slow.

Distinct From Conventional Drugs

Common medicines such as aspirin, antacids and statins are chemical in nature. Though many were initially discovered in the wild (aspirin is a cousin of a compound in willow bark, the first statin was found in a fungus), these drugs are now made nonbiologically.

Conventional medicines are stitched together by chemists in large factories using other chemicals as building blocks. Their molecular structures are well defined and relatively simple. Aspirin, for example, contains just 21 atoms (nine carbons, eight hydrogens and four oxygens) bonded together to form a particular shape. A single aspirin tablet even kid-sized contains trillions of copies of the drug molecule.

Biologic drugs are a different story. This class of medication is not synthesized chemically instead they are harvested directly from biology, as their name suggests. Most modern biologics are assembled inside vats or bioreactors that house genetically engineered microbes or mammalian cell cultures. Efforts are underway tomake them in plants.

Biologic drugs can bewhole cells, alive or dead. They can be the biomolecules produced by cells, likeantibodies, which are normally secreted by our immune system's B cells. Or they can be some of the internal components of cells, likeenzymes.
Biologics can dwarf conventional drugs in size, and that gives them added specificity.Ian Haydon,CC BY-ND

Biologics are typically much larger molecules than those found in conventional pharmaceuticals, and in many cases their exact composition is unknown (or even unknowable). You're unlikely to find biologic drugs in tablet form they tend to be delicate molecules that are happiest in liquid solution.

While biologics are one of thefastest-growing drug categoriesin the U.S., they aren't exactly new. TheBiologics Control Act, passed in 1902, was the first law aimed at ensuring the safety of some of the earliest biologics vaccines. Congress was moved to pass the law after a contaminated batch of diphtheria shots left 13 children dead. Jim, the horse from which the diphtheria antitoxin had been extracted,had contracted tetanus.

Fortunately, scientists have dramaticallyimproved the way they manufacturebiologic drugs since then. For starters, the recombinant DNA revolution of the 1970s means that drug makers no longer have to extract many of the most important biologics from whole animals.

The gene that codes for human insulin, for example, can be pasted into a microbe which will happily churn out the drug in bulk. After amultimillion dollar purification process, the injectable insulin that resultsis indistinguishablefrom the version a healthy human body would produce. This is how some forms of insulin are made today.

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Immunotherapy: Training the Body to Fight Cancer

In animal models and clinical trials, breakthrough immunotherapies are emerging.

The Biologic Advantage

Both conventional and biologic drugs work by interacting with our own biology. Most conventional drugs function as inhibitors they're just the right size and shape to jam themselves into some molecular cog in our cells. Aspirin's pain-reducing power comes from itsability to disrupt an enzymein the body called cyclooxygenase, an important player in pain signaling.

Conventional drug discovery largely consists of finding new compounds that specifically disrupt only disease-associated processes. Because these drugs are quite small, and because the inside of any cell is a sea of other molecular components, finding a new small drug that blocks only problematic processes is tricky. Off-target interactions canproduce side effectsof all types.

The large size of biologic drugs can be an asset here. An antibody, for example, has lots of specific points of contact with its target. This enables therapeutic antibody drugs to bind with extreme precision only their target molecule should be an exact match. This binding can lead to inhibitory effects, much like a conventional drug might. In some cases, therapeutic antibodies can alsostimulate the immune systemin a problem area, like at a tumor, prompting the body totake it out.

Many biologics target molecular processes that no conventional drug can, and they can treata growing list of diseases. Cancer treatmentsdominate the list, but since 2011 the U.S. Food and Drug Administration has approved new protein-based biologics for the treatment of Lupus, Crohn's disease, rheumatoid arthritis, multiple sclerosis, kidney failure, asthma and high cholesterol.

New types of biologic drugs continue to emerge as well. In late 2015, the FDA approved a first-of-its-kind treatment for patients with advancedmelanoma: an engineered herpes virus. Researchers genetically programmed the virus, calledT-VEC, to target only cancerous cells, and it can also prompt the immune system to start wiping out cancer.Additional virus-based therapiesare currently working their way through the lengthy U.S. drug approval process.
Crippling Costs

Amgen, the company that produces T-VEC, estimates it will cost an average of65,000 per patient and that doesn't come close to topping the list of priciest biologic medications. The most expensive drug ever made recentlywon approvalby the FDA. Brineura, a biweekly enzyme replacement therapy produced by BioMarin Pharmaceutical, delays the loss of walking in individuals with a rare genetic disorder. Its price tag? 27,000 per injection, ormore than 700,000for a full year's treatment.

The steep prices of biologic drugs are alarming to many patients, physicians and researchers. In an effort to drive costs down, provisions of the Obama administration'sAffordable Care Actaccelerated the approval process for new biologics intended to compete with already approved medicines. Like generic drugs, so-called biosimilars are designed to be interchangeable with the biologic they seek to replace.

Unlike generic versions of conventional drugs, however, biosimilar drugs are often only similar to not identical with their competition. This means these complex drugs still require lengthy and expensive trials of their own to make sure they're effective and safe. Because of this, the Federal Trade Commission estimates that biosimilars may only produce an overall10 to 30 percent discountfor patients.

Cost-cutting innovations in the biologic production pipeline are desperately needed. The FDA hascalled on scientists and drug developersto invent biosimilars that resemble FDA-approved medicines and to develop the tools needed to quickly demonstrate their safety.

As this promising class of drugs continues to grow in number and popularity, their lifesaving power will be limited if costs make them inaccessible to patients who need them.

This article was written byIan Haydon, doctoral student in biochemistry,University of Washington, forThe Conversation. It has been republished with permission.

Original Source:https://www.usnews.com/news/healthcare-of-tomorrow/articles/2017-07-25/biologics-the-drugs-that-are-transforming-medicine

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Detroit Red Wings say farewell to Joe Louis Arena, close a chapter

Jeff Seidel , Detroit Free Press Columnist

They screamed and raised countless cups of beer and held up thousands of cell phones, trying to record this moment, as the final seconds ticked down in Joe Louis Arena three, two, one.

And it was over.

An octopus came flying onto the ice and a red light swirled over the crowd, as the Detroit Red Wings skated around the Joe, one last time. The players raised up their sticks, applauding the fans, as Auld Lang Syne blared over the loudspeakers.

Farewell, Joe.

I dont think I've never had goosebumps this many times in a game, captain Henrik Zetterberg said, after the Wings played their final game in this old arena along the riverfront, a 4-1 victory over the New Jersey Devils that turned into a wild, deafening, rollicking, sing-along.

This was more than a farewell to a hockey rink. This was like closing a door to your childhood, for both the players and fans. You remember the rink you grew up on, former forward Darren McCarty said. For most of us, this is the one we remember.

This was one last chance to savor the memories. The Cups. The quirks. The beer. The banners. The brawl. The blood. The octopi. The champagne. The chants. The smells oh, the smells. And that 25-year playoff streak, which came to an end along with this arena on Sunday afternoon.

Sure, this place might be ugly. It might not make sense by todays standards. Built with no press box. Designed with no windows facing the river. Along with the cramped concourse and scarce bathrooms. But it was our ugly.

It's been an awesome building to play in, Steve Yzerman said, just off the ice, wearing a Wings jersey once again, waiting for the postgame ceremony. Tremendous memories. But the new building will be even better. It will take the Red Wings to another level.

Early chills

The goosebumps started before the game.

Thousands of Wings fans, all of whom were born and raised in South Detroit, of course, stood on the steps, facing the Detroit River, and lined Steve Yzerman Drive seven deep in places, spilling down the grassy bank to the sidewalk and climbed up on barriers, and raised up on tippy toes along the red carpet, trying to catch a glimpse of the Red Wings.

At its core, this building was about relationships a city and a team, fathers and mothers, sons and daughters, passing a love of hockey, no, more than that, a love of this team from generation to generation.

That's why so many players came back for this game.

That's why Adam Beauregard stood in the hallway outside a suite, holding his 6-week-old son, Finn, who was given a certificate for attending his first Red Wings game.

"Had to be here," said Beauregard, 31, of Saginaw. "I got tickets last night."

Beauregard bounced up and down, trying to keep Finn content. "He likes it so far. I grew up my whole life, playing hockey, watching hockey and I'm planning the same thing for him."

A milestone

Before the game, down on the ice, Zetterberg was honored for playing in his 1,000th Red Wings game. How incredibly fitting , and a sea of red and white stood up and cheered.

Then, Yzerman came down the red carpet for the ceremonial puck drop.

Ste-vie! the fans chanted. Ste-vie! Ste-vie!

It sounded like echoes from another time, coming down from the ceiling.

"It sounded wonderful. Yeah, its pretty nice," Yzerman said. "I'm not gonna lie."

This turned into a massive reunion weekend, as about 65 former players came back to say farewell, and every moment seemed better than the last.

The last time that Karen Newman came out and sang the national anthem. As she finished, somebody chucked an octopus onto the ice. Then, here came another. And another.

"Lets go, Red Wings!" the fans chanted.

"Look around and savor it. The banners. The red plastic seats. At some point, you will get choked up," McCarty said. "How can you not? It's like you are putting down your 15-year-old golden retriever. You don't want to see it suffer."

McCarty's favorite memory was smoking a cigar on a golf cart, riding through the bowels of the arena, from the dressing room to a news conference after winning the '97 Cup. "I remember the smell, you can't forget the smell," he said. "The whole hockey. Springtime. Humidity. Sweat. Champagne. Whatever."

Relishing the moment

"Listen, I can't wait for the new arena, selfishly having a functional press box will be incredible. But at some point on Sunday afternoon, I started wishing: Don't let this endgame end too fast."

Maybe it was after Zetterberg scored, giving the Wings a 3-0 lead, and an octopus slid across the ice, followed by, what looked like, a baby one. A song blared over the loudspeakers 'A-B-C. It's easy as 1, 2, 3,' and there was Al Sobotka, waving another octopus.

Or maybe, it was when an alumni ice crew came out, a group of guys who need only one name by this crowd: Kocur, McCarty, Holmstrom, Murphy and Ciccarelli. And there was Sobotka, waving another octopus. Good grief. His arm is gonna be sore.

Or maybe, it was the sounds and music that turned into a giant sing-along.

"Josie's on a vacation far away.

"Oh! Halfway there."

Or maybe, it was when the music ended and the crowd kept signing, a cappella. "Please tell me why. My car is in the front yard. And I am sleeping with my clothes on."

Or maybe, it was the sign somebody held up and was shown on the video screen: Lemieux turtled.

We pause here to smile.

The fans mattered

Now, a word about the Red Wings fans. Because this team would be nothing without these die-hard fans, and Ryan Ives might just symbolize all of them.

It's not the most beautiful barn in the world, but it is to me, Ives said.

He wore a Red Wings mask and a Red Wings cap, not to mention Wings socks and a Dylan Larkin jersey over a Niklas Kronwall jersey. Yes,he was wearing two jerseys which covered the Red Wings tattoo down his right arm.

The Wings mean everything to me, said Ives, who grew up across the river in Leamington, Ontario a huge Wings fan, just like his father before him. When I think about the Red Wings, I think about my childhood, all the good memories with them, sitting around the house, with the old man and the mum, eating munchies, eating wings, watching the Wings play. Whether it was the good times 97, 98 or the bad times.

Now, he lives in Calgary, and he took a four-hour flight on Saturday from Calgary to London, Ontario, and then a two-hour drive to Detroit, just to see this game.

We had to come here for this game, said Ives, who dropped about 3,000 for the tickets, airfare and hotel. But money doesnt matter. We love the Wings. It means everything. Im getting emotional, just talking about it.

But now?

The Wings will head to Little Caesars Arena.

The new place is new hope, new adventures, Ives said. We got a brand-new team coming. There arent many of them left. Its all the new young guys. The streak has died with the Joe. Come on. Lets start a new streak.

The memories

What happens when your goosebumps get goosebumps?

That's what the post-game ceremony was like, a trip through the memories and a Red Wings museum come to life.

VLADDIE! VLADDIE! VLADDIE!

Ozzie! Ozzie! Ozzie!

Scotty! Scotty! Scotty!

The long, genuine, heartfelt standing ovation for the Ilitch family still grieving the loss of Mr. I.

The warm speech by Yzerman.

"Come home, Stevie!" the fans chanted. "Come home, Stevie!"

After the speeches and videos, it ended the only way it could end, with everybody singing together: Just a city boy. Born and raised in South Detroit.

Sticks shot in the air. Lights swirled. The air filled with smoke.

The lights came up, the music kept playing, and the fans kept standing at their seats. Taking pictures. Looking around.

It was like nobody wanted to leave.

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(April 7, 2017) Innovative Research's Normal Single Donor Human Plasma was recently used in the following study: 

Carcinogenic Activities and Sperm Abnormalities of Methicillin Resistance Staphylococcus aureus and Inhibition of Their Virulence Potentials by Ayamycin

Mervat Morsy Abbas Ahmed El-Gendy, Khaled G. Abdel-Wahhab, Fathia A. Mannaa, Ayman A. Farghaly, Ahmed M. A. El-Bondkly
... Twenty microliters of each culture supernatant was added to 100 L phosphate buffered saline (PBS), 10 L human plasma (Innovative Research, Novi, MI), and 10 L S2403 (1 mg mL 1 , Diapharma group Inc ...

(April 3, 2017) Innovative Research's Normal Single Donor Human Plasma was recently used in the following study: 

Molecular impact of selective NFKB1 and NFKB2 signaling on DLBCL phenotype

X Guo, J L Koff, A B Moffitt, M Cinar, S Ramachandiran, Z Chen, J M Switchenko, M Mosunjac, S G Neill, K P Mann, M Bagirov, Y Du, Y Natkunam, H J Khoury, M R Rossi, W Harris, C R Flowers, I S Lossos, L H Boise, S S Dave, J Kowalski and L Bernal-Mizrachi...
... The ABC-DLBCL lines OCI-LY10 and OCI-LY3 and the GCB-DLBCL lines OCI-LY1, OCI-LY2 and OCI-LY19 were maintained in Iscove's medium supplemented with 20% fresh human plasma (Innovative Research, Novi, MI, USA), 1% L-glutamine, 1 mM sodium pyruvate and 50 ...

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(March 31, 2017) Innovative Research's Mouse Prealbumin ELISA Kit was recently used in the following study: 

Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4

Mei Du, Ashley Martin, Franklin Hays, Jennifer Johnson, Rafal A. Farjo, Krysten M. Farjo, 2017...
... ELISA kits for mouse TTR (catalog no IRKTAH1161; Innovative Research, Novi, MI), mouse RBP4 (catalog no MRB400; R&D Systems, Minneapolis, MN), and human RBP4 (catalog no DRB400; R&D Systems) were used to quantify target molecules in serum and retina lysates. ...

(March 17, 2017) Innovative Research's Pooled Normal Human Urine and Single Donor Human Whole Blood were recently used in the following study: 

Detection of Chemical Warfare Agent Simulants and Hydrolysis Products in Biological Samples by Paper Spray Mass Spectrometry

J McKenna, ES Dhummakupt, T Connell, P Demond... - Analyst 2017...Scientific (Fair Lawn, NJ, USA).
Raw human urine and human whole blood with K2EDTA anticoagulant was purchased from Innovative Research (Novi, MI, USA). ......

(March 20, 2017) Innovative Research's Pooled Normal Human Urine was recently used in the following study: 

A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

A Priye, SW Bird, YK Light, CS Ball, OA Negrete... - Scientific Reports, 2017
... or urine) to various virus concentrations. Human sample matrices pooled from de-identified healthy donors were purchased from a commercial supplier (Innovative Research, Novi, MI). The matrices were tested by the supplier...

(March 27, 2017) Innovative Research's Heat-Inactivated Human Serum was recently used in the following study: 

Two-photon polymerization for production of human iPSC-derived retinal cell grafts

KS Worthington, LA Wiley, EE Kaalberg, MM Collins - Acta Biomaterialia, 2017
... ... dissociated and resuspended individual iPSC colonies derived from a donor with normal ocular history in 3D differentiation medium DMEM (Gibco, Thermo Fisher Scientific, Grand Island, NY), 10% heat-inactivated human serum (Innovative Research, Novi, MI).

(March 30, 2017) Innovative Research's Single Donor Human Serum was recently used in the following study: 

Comparison of O-polysaccharide and hemolysin co-regulated protein as target antigens for serodiagnosis of melioidosis

A Pumpuang, SJ Dunachie, P Phokrai, K Jenjaroen - PLOS Neglected Tropical , 2017
... who were admitted to Sappasithiprasong hospital, Ubon Ratchathani, northeast Thailand, 188 serum samples obtained from healthy donors from the same area in northeast Thailand and 90 serum samples obtained from healthy US donors (Innovative Research, Novi, MI, USA ...

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INNOVATIVE RESEARCH PRODUCT REFERENCES - FEBRUARY 2017

Written by jessica on April 19, 2017. Posted in Blog

(February 28, 2017) Innovative Research's Pooled Normal Human Plasma was recently used in the following study:  Practical applications of infrared, Raman and nuclear magnetic resonance spectroscopic techniques for qualitative, quantitative and structural analysis of H Jakupovi - 2016...
Pooled normal human plasma was purchased from Innovative Research, Inc., (Novi, MI, USA). Deionized water for all the experiments was...



(February 21, 2017) Innovative Research's Bovine Red Blood Cells, Packed 100% was recently used in the following study: 

Antifungal Efficacy of an Intravenous Formulation Containing Monomeric Amphotericin B, 5-Fluorocytosine, and Saline for Sodium Supplementation

C Alvarez, DR Andes, JY Kang, C Krug, GS Kwon - Pharmaceutical Research
...The experiment was performed in triplicate. Hemolysis Assay. To determine the hemolytic activity of different formulations, bovine red blood cells (Innovative Research, Novi, MI) were washed three times and diluted 200-fold...



(February 21, 2017) Innovative Research's Pooled Normal Human Plasma and Innovative Grade US Origin Sprague Dawley Rat Plasma were recently used in the following study: 

Stability studies of potent opioid analgesic, morphine 6O sulfate in various buffers and biological matrices by HPLCDAD analysis

JSK Yadlapalli, ZAF Albayati, NR Penthala - Biomedical , 2017
...cardiovascular effects, constipation, and hyperalgesia. It has been shown by many researchers that structural modification of the 3-O- ... standards (USP). Rat and Human plasma were purchased from Innovative research, Novi, MI. ...



(February 21, 2017) Innovative Research's Sheep Red Blood Cells, Packed 10% was recently used in the following study: 

Role of STARD4 in sterol transport between the endocytic recycling compartment and the plasma membrane

DB Iaea, S Mao, FW Lund, FR Maxfield - Molecular Biology of the Cell, 2017
... membrane. MATERIALS AND METHODS Materials: Sheep red blood cells were purchased from Innovative Research Inc. (Novi, MI). ...



(February 14, 2017) Innovative Research's Chicken Red Blood Cells, Packed 5% was recently used in the following study:

Genome scaffolding and annotation for the pathogen vector Ixodes ricinus by ultra-long single molecule sequencing...

WJ Cramaro, OE Hunewald, L Bell-Sakyi, CP Muller - Parasites & Vectors, 2017
... Methods. Genome size determination by flow cytometry. Labeling and fixation of cells. Primary chicken red blood cells (CRBCs; Innovative Research Inc., Novi, MI, USA)



(February 14, 2017) Innovative Research's Pooled Normal Human Plasma was recently used in the following study: 

Targeted proteomics and absolute protein quantification for the construction of a stoichiometric host-pathogen surface density model

K Sjöholm, O Kilsgård, J Teleman, L Happonen… - Molecular & Cellular …, 2017
... Plasma adsorption Human blood plasma (pooled healthy human plasma Na-citrate, Innovative Research, Novi, MI, USA) was mixed with bacteria, in a ratio 3:1, and the samples were incubated for 30 minutes at 37°C, allowing plasma proteins to adsorb to the bacterial surface...

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(January 19, 2017) Innovative Research's Innovative Grade US Origin Sprague Dawley Rat Serumwas recently used in the following study:

Chiral determination of 3, 4-methylenedioxypyrovalerone enantiomers in rat serum

MD Hambuchen, H Hendrickson, SM Owens - Analytical Methods, 2016 ... Innovative Research (Novi, MI) at concentrations of 3, 100, and 1600 ng/ml to allow for analysis of (R)- and (S)-enantiomer concentrations of 1.5, 50, and 800 ng/ml. 3,4- ... serum from Innovative Research (Novi, MI), male and female SD rat serum from...



(January 19, 2017) Innovative Research's Pooled Normal Human AB Serum was recently used in the following study:

Determination of Renal Function and Injury Using Near-Infrared Fluorimetry in Experimental Cardiorenal Syndrome

M Ikeda, R Wakasaki, KJ Schenning, T Swide, JH Lee - American Journal of , 2017 ZW800-1 samples were diluted in phosphate-buffered saline (control) or mouse serum 151 (Innovative Research, Novi, MI) and incubated at 37C for 4h (n=16). Fluorescence was 152 measured hourly on a plate reader and expressed as percent of baseline fluorescence....



(January 10, 2017) Innovative Research's Innovative Grade US Origin Porcine Whole Bloodwas recently used in the following study:

Identification and Characterization of Glycopeptides from Egg Protein Ovomucin with Anti-Agglutinating Activity against Porcine K88 Enterotoxigenic Escherichia coli

X Sun, MG Gnzle, J Wu - Journal of Agricultural and Food Chemistry, 2017.
...Porcine whole blood 82 was bought from Innovative Research Inc. (Novi, MI, USA). Milli-Q water was 83 prepared by the Milli-Q water supply system



(January 9, 2017) Innovative Research's Pooled Normal Human AB Serum was recently used in the following study:

The lack of natural processes of delivery and neonatal intensive care treatment lead to impaired cytokine responses later in life

MV Martikainen, L KeskiNisula, H Jakupovi- American Journal of , 2017 ... Louis, USA). For functional studies, PBMCs were thawed and resuspended at 110 6 viable cells/mL in RPMI 1640 supplemented with 10% human AB serum (Innovative Research, Novi, MI, USA), and 1% L-glutamine and 1% antibiotic/antimycotic ...



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INNOVATIVE RESEARCH PRODUCT REFERENCES - FALL 2016

Written by jessica on April 19, 2017. Posted in Blog

(November 22, 2016) Innovative Research's Innovative Grade US Origin Cyno Monkey Serum was recently used in the following study:

Complement inhibition enables tumor delivery of LCMV glycoprotein pseudotyped viruses in the presence of antiviral antibodies

L Evgin, CS Ilkow, MC Bourgeois-Daigneault - Molecular Therapy , 2016... postadministration). As described with rat and mouse immune serum, neutralization was assessed following incubation of HI immune serum (1 hour; 37 C) GVB or with cynomolgus macaque serum (Innovative Research, Novi, MI)...



(November 21, 2016) Innovative Research's Premium US Origin Fetal Bovine Serumwas recently used in the following study:

Detection of drugresponsive Blymphocytes and antidrug IgG in patients with lactam hypersensitivity

MO Amali, A Sullivan, RE Jenkins, J Farrell, X Meng - Allergy, 2016...100mM L-glutamine, 100 g/ml penicillin, and 100 U/mL streptomycin. FBS and human AB serum were purchased from Innovative Research (Novi, MI, USA), respectively. All other culture reagents were purchased from...



(November 15, 2016) Innovative Research's Pooled Normal Human Serum was recently used in the following study:

Characterisation of successive Acinetobacter baumannii isolates from a deceased haemophagocytic lymphohistiocytosis patient

HJ Choi, MC Kil, JY Choi, SJ Kim, KS Park, YJ Kim - International Journal of , 2016... 2.6. Serum sensitivity assay. Susceptibility to 40% normal human serum (NHS) (Innovative Research, Inc., Novi, MI) was determined using a previously described method 13 with modifications. Serum susceptibilities were determined...



(November 7, 2016) Innovative Research's Innovative Grade US Origin Mouse Plasma -CD-1 was recently used in the following study:

S2subsite variations between human and mouse enzymes (plasmin, factor XIa, kallikrein) elucidate inhibition differences by TFPI2 domain1wildtype, Leu17Arg

K Vadivel, Y Kumar, GI Ogueli, SM Ponnuraj - Journal of Thrombosis and.. Materials and methods Materials Citrated mouse plasma was from Innovative Research (Novi, MI)



(October 24, 2016) Innovative Research's Innovative Grade US Origin Donkey Plasma was recently used in the following study:

Cross Spe-cies applicability of Anti-human plasma IgY affinity column.(2016) J Immune Serum Biol 2 (1): 1-5

SB Mark - Research article, 2016
... (Irvine CA). Donkey plasma (IDK-N) was purchased from Innov-Research, Inc. (Novi, Michigan).



(September 27, 2016) Innovative Research's Rat Albumin ELISA Kit was recently used in the following study:

Consumption of Dietary Resistant Starch Partially Corrected the Growth Pattern despite Hyperglycemia and Compromised Kidney Function in Streptozotocin-induced

GY Koh, MJ Rowling, K Schalinske, K Grapentine - 2016...Rat albumin ELISA kits were obtained from Innovative Research (Novi, 96 MI)...25-hydroxycholelcalciferol 97



(September 25, 2016) Innovative Research's Single Donor Human Red Blood Cells - Washed was recently used in the following study:

Cathelicidin Antimicrobial Peptides with Reduced Activation of Toll-Like Receptor Signaling Have Potent Bactericidal Activity against Colistin-Resistant Bacteria

C Kao, X Lin, G Yi, Y Zhang, DA Rowe-Magnus, K Bush - mBio, 2016.... cells (hRBCs) (catalog no. IPLA-WB3-18103; Innovative Research, Inc., Novi, MI). The hRBCs were washed three times with phosphate-buffered saline (PBS) (pH 7.4) and then resuspended in PBS. hRBC solution was mixed ...

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(November 15, 2016) Innovative Research's Pooled Normal Human Serum was recently used in the following study:

Characterisation of successive Acinetobacter baumannii isolates from a deceased haemophagocytic lymphohistiocytosis patient

HJ Choi, MC Kil, JY Choi, SJ Kim, KS Park, YJ Kim - International Journal of , 2016... 2.6. Serum sensitivity assay. Susceptibility to 40% normal human serum (NHS) (Innovative Research, Inc., Novi, MI) was determined using a previously described method 13 with modifications. Serum susceptibilities were determined



(November 7, 2016) Innovative Research's Innovative Grade US Origin Mouse Plasma -CD-1 was recently used in the following study:

S2-subsite variations between human and mouse enzymes (plasmin, factor XIa, kallikrein) elucidate inhibition differences by TFPI-2 domain1-wildtype, Leu17Arg

K Vadivel, Y Kumar, GI Ogueli, SM Ponnuraj - Journal of Thrombosis and.. Materials and methods Materials Citrated mouse plasma was from Innovative Research (Novi, MI)



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(January 2, 2016) Innovative Research's Pooled Normal Human Plasma was recently used in the following study:

Targeted gene transfer to the brain via the delivery of brain-penetrating DNA nanoparticles with focused ultrasound

BP Mead, P Mastorakos, JS Suk, AL Klibanov, J Hanes - Journal of Controlled , 2015
... Effective shielding of the NP positive surface charge was confirmed by the near-neutral -potential ( 1.5 0.3 mV; Table 1). We further measured the stability of DNA-BPN in pooled human plasma (PHP; Innovative Research, Novi, MI); DNA-BPN retained their colloidal stability ...



(January 2, 2016) Innovative Research's Innovative Grade US Origin Sprague Dawley Rat Plasma was recently used in the following study:

Determination of pinostilbene in rat plasma by LC-MS/MS: Application to a pharmacokinetic study

W Chen, SCM Yeo, XF Chuang, HS Lin - Journal of Pharmaceutical and Biomedical , 2015
... Pooled Sprague-Dawley rat plasma was supplied from Innovative Research (Novi, MI, USA) and used as blank matrix in the preparation of calibration standards and QC samples. 2.3. Preparation of calibration standards and QC samples. ...



(January 5, 2016) Innovative Research's Pooled Normal Human Plasma was recently used in the following study:

Multiplex Preamplification of Serum DNA to Facilitate Reliable Detection of Extremely Rare Cancer Mutations in Circulating DNA by Digital PCR

JB Jackson, DS Choi, JD Luketich, A Pennathur - The Journal of Molecular , 2016
... Extraction of ccfDNA from pooled normal human plasma (wild-type control; Innovative Research, Novi, MI) and patient serum (1 to 3 mL per patient) was performed...



(January 5, 2016) Innovative Research's Pooled Normal Human Plasma was recently used in the following study:

Endomorphin Analog Analgesics with Reduced Abuse Liability, Respiratory Depression, Motor Impairment, Tolerance, and Glial Activation

JE Zadina, MR Nilges, J Morgenweck, X Zhang - Neuropharmacology, 2015
... To test analog degradation by human and rat plasma enzymes in vitro, pooled normal human plasma was obtained from Innovative Research (Novi, MI, cat.IPLA-N) and rat aortic blood was drawn in a 10ml syringe rinsed with heparin, incubated at room temperature 1 hr and ...



(January 9, 2016) Innovative Research's Single Donor Human Whole Blood was recently used in the following study:

PDF Direct Biofluid Analysis using Hydrophobic Paper Spray Mass Spectrometry

DE Damon, KM Davis, CR Moreira, PC Capone - Analytical Chemistry, 2016
... Human blood was purchased from Innovative Research (Novi, MI). Phosphate buffered saline (PBS) tablets were purchased from AMRESCO (Solon, OH). ...



(January 24, 2016) Innovative Research's Pooled Normal Human Urine was recently used in the following study:

HTML Validation of reliable and selective methods for direct determination of glyphosate and aminomethylphosphonic acid in milk and urine using LC-MS/MS

PK Jensen, CE Wujcik, MK McGuire, MA McGuire - Journal of Environmental Science , 2016
... Institutional Review Boards. Pooled normal human urine was obtained from Innovative Research (Novi, MI, USA). At least three unique pools of each matrix were used for method validation. Preparation of analytical standards. ...



(January 26, 2016) Innovative Research's Single Donor Human Serum was recently used in the following study:

HTML Cross-kingdom inhibition of breast cancer growth by plant miR159

AR Chin, MY Fong, G Somlo, J Wu, P Swiderski, X Wu - Cell Research, 2016
...and subjected to reverse transcription (RT)-qPCR for miR159 and miR-16 as described 7 . Individual and pooled healthy female donor human sera were purchased from Innovative Research (Novi, MI). ...

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(11-3-15) Innovative Research's Pooled Complement Human Serum was recently used in the following study:

Antibodies produced by clonally expanded plasma cells in multiple sclerosis cerebrospinal fluid cause demyelination of spinal cord explants

K Blauth, J Soltys, A Matschulat, CR Reiter, A Ritchie - Acta Neuropathologica, 2015
... rAbs derived from MS patients and neuroinflammatory controls were applied to the media for 72 h at concentrations of 50 g/mL in the presence and absence of 10 % human complement (hComp) (pooled nor- mal human complement serum; Innovative Research, Novi, MI, USA ...



(11-3-15) Innovative Research's Single Donor Human Serum was recently used in the following study:

Liquid chromatography-tandem mass spectrometric assay for the simultaneous determination of the irreversible BTK inhibitor ibrutinib and its dihydrodiol-metabolite in

JJM Rood, S van Hoppe, AH Schinkel, JHM Schellens - Journal of Pharmaceutical , 2015
... West Sussex, UK). For the evaluation of selectivity and matrix effect, human lithium-heparinized plasma of six individual donors were obtained from Innovative Research (Novi, MI, USA). 2.2.



(11-7-15) Innovative Research's Human Resistin ELISA Kit was recently used in the following study:

PDF Monocyte chemoattractant protein-1, trans-forming growth factor-1, nerve growth factor, resistin and hyaluronic acid as serum markers: comparison between

MG Kamath, CG Pai, A Kamath, A Kurien - 2015
... The serum markers were determined by using commercially available ELISA kits for MCP-1 (Yes Biotech Laboratories Ltd., Canada), TGF-1 (Yes Biotech Labo- ratories Ltd.), NGF (Signosis, Inc., Sunnyvale, CA, USA), Resistin (Innovative Research, Novi, MI, USA) and HA ...



(11-7-15) Innovative Research's Sheep Anti Rat & Mouse Renin IgG Fraction was recently used in the following study:

PDF A single simple procedure for dewaxing, hydration and heat-induced epitope retrieval (HIER) for immunohistochemistry in formalin fixed paraffin-embedded tissue

IMS Paulsen, H Dimke, S Frische - European Journal of Histochemistry, 2015
... 14 pNKCC2: H934, a custom made rabbit poly- clonal antibody specifically recognizing a double phosphorylated form of the furosemide sensitive sodium, potassium, 2 chloride co-transporter, NKCC2.9 Renin: ISASRREN-GF, (Innovative Research, Novi, MI, USA), sheep ...



(11-7-15) Innovative Research's Innovative Grade US Origin Bovine Whole Blood was recently used in the following study:

Paper-capillary spray for direct mass spectrometry analysis of biofluid samples

Y Ren, S Chiang, W Zhang, X Wang, Z Lin, Z Ouyang - Analytical and Bioanalytical
... Louis, MO, USA). Bovine whole blood was purchased from Innovative Research (Novi, MI, USA). Chromatography papers (Grade 1 and 31ET) used for making paper substrates were purchased from Whatman (Whatman International, Maidstone, UK). ...



(11-23-2015) Innovative Research's Human Lactoferrin ELISA Kit was recently used in the following study:

Innate Immune Factors in Mothers' Breast Milk and Their Lack of Association With Rotavirus Vaccine Immunogenicity in Nicaraguan Infants

S Becker-Dreps, WS Choi, L Stamper, S Vilchez - Journal of the Pediatric , 2015
... where serum samples were separated and then stored at 70C and breast milk samples were stored at 70C. Commercial kits were used to assay breast milk concentrations of lactoferrin (Innovative Research Inc, Novi, MI)...



(11-23-15) Innovative Research's Pooled Normal Human Serum was recently used in the following study:

Globally Optimized Targeted Mass Spectrometry (GOT-MS): Reliable Metabolomics Analysis with Broad Coverage

H Gu, P Zhang, J Zhu, D Raftery - Analytical Chemistry, 2015
... To develop GOT-MS, pooled human serum samples were purchased from 106 Innovative Research, Inc. (Novi, MI). For the CRC metabolomics study, serum samples 107 from 18 CRC patients and 20 healthy controls were selected from those involved in our 108 ...



(11-23-15) Innovative Research's Normal Human Breast Milk was recently used in the following study:

PDF Sensing Small Changes in Protein Abundance: Stimulation of Caco-2 Cells by Human Whey Proteins

JK Cundiff, EJ McConnell, KJ Lohe, S Maria - of Proteome Research, 2015
... Human breast milk was from Innovative Research, Inc. (Novi, MI) and the whey proteins (WP) extracted as previously described. ...



(11-27-15) Innovative Research's Mouse Serum was recently used in the following study:

PDF Identification and Characterization of the Trypanosoma cruzi B-cell Superantigen Tc24

SM Gunter, KM Jones, B Zhan, HT Essigmann - The American Journal of , 2015
... cardiac puncture under anesthesia (150 mg/kg ketamine/15 mg xylazine). Pooled sera from noninfected and noninjected Balb/c, C57BL/6, CD-1, and non-Swiss albino mice were also obtained from Innovative Research (Novi, MI). ...



(11-27-15) Innovative Research's Innovative Grade US Origin Sprague Dawley Rat Plasma was recently used in the following study:

Simultaneous determination of 3-mercaptopyruvate and cobinamide in plasma by liquid chromatography-tandem mass spectrometry

MW Stutelberg, JK Dzisam, AR Monteil, I Petrikovics - Journal of Chromatography , 2015
... Cbi as a cyanide therapeutic in rats. Rat plasma (with Na Heparin as an anti-coagulant) was purchased from Innovative Research (Novi, MI) and stored at -80 C until used....



(12-17-15) Innovative Research's Sheep Red Blood Cells, Packed 100% was recently used in the following study:

Tolerogenic nanoparticles to induce immunologic tolerance: Prevention and reversal of FVIII inhibitor formation

AH Zhang, RJ Rossi, J Yoon, H Wang, DW Scott - Cellular Immunology, 2015
... Sheep red blood cells (SRBC) were purchased from Innovative Research, Inc., (Novi, MI)...



(12-22-15) Innovative Research's Pooled Normal Human Plasma was recently used in the following study:

Development and validation of a highly sensitive LCMS/MS method for the determination of acacetin in human plasma and its application to a protein binding study

SB Kim, T Lee, HS Lee, CK Song, HJ Cho, DD Kim - Archives of Pharmacal Research
... Sang-Bum KimAffiliated withNew Drug Development Center, DaeguGyeongbuk Medical Innovation Foundation; , Taehun LeeAffiliated withCollege of ... Pooled normal human plasma (from donors aged 1865 years) was purchased from Innovative Research (Novi, MI, USA). ...



(12-29-15) Innovative Research's Mouse Prorenin 8x His was recently used in the following study:

Activation of ENaC in Collecting Duct Cells by Prorenin and Its Receptor PRR: Involvement of Nox4-Derived Hydrogen Peroxide

X Lu, F Wang, M Liu, KT Yang, A Nau, DE Kohan - American Journal of , 2015
... 98 Mouse prorenin (IMPREN-HIS) purchased from Innovative Research (Novi, MI, USA) ...

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September and October 2015 References

Written by admin on March 30, 2016. Posted in Blog

Innovative Research's Pooled Normal Human AB Serum was recently used in the following study:

(9-17-15)

Exposure to a farm environment is associated with T helper 1 and regulatory cytokines at age 4.5 years

H Kaario, K Huttunen, AM Karvonen, B Schaub - Clinical & Experimental , 2015
... Louis, USA). Mean concentration of live PBMCs/stimulation was 0.8 106/ml (SD 0.3 106/ml). PBMCs were cultivated in 1 mL of 10 % human AB serum (Innovative Research, Novi, MI, USA) in RPMI 1640 at 37 C and 5 % CO2 for 5 hours. Cytokine analysis ...



Innovative Research's Pooled Normal Human Plasma was recently used in the following study:

(9-18-15)

Nanoscale plasma coating inhibits formation of Staphylococcus aureus biofilm

Y Xu, JE Jones, H Yu, Q Yu, GD Christensen, M Chen - Antimicrobial Agents and , 2015
... The sterilized coupons were then coated by overnight incubation in 135 20% (v/v) dilution of human plasma (Innovative Research, Novi, MI) in phosphate-buffered 136 saline (PBS). Coupons coated with human plasma were incubated in 1 ml Todd-Hewitt broth 137 ...



Innovative Research's Mouse PAI-1 ELISA Kit was recently used in the following study:

(10-5-15)

Neutralization of the antifibrinolytic function of plasminogen activator inhibitor1 resolves skin fibrosis

R Lemaire, T Burwell, H Sun, T Delaney, J Bakken - Arthritis & Rheumatology, 2015
... Mouse active PAI-1 and active plasmin levels were determined in plasma and skin lysates using ELISA kits as instructed by manufacturer (Innovative Research (Novi, Michigan). RNA analysis. Murine skin tissues were homogenized using a Polytron (Kinematica, Bohemia, ...



Innovative Research's Mouse Plasminogen Total ELISA Kit was recently used in the following study:

(10-5-15)

Neutralization of the antifibrinolytic function of plasminogen activator inhibitor1 resolves skin fibrosis

R Lemaire, T Burwell, H Sun, T Delaney, J Bakken - Arthritis & Rheumatology, 2015
... Mouse active PAI-1 and active plasmin levels were determined in plasma and skin lysates using ELISA kits as instructed by manufacturer (Innovative Research (Novi, Michigan). RNA analysis. Murine skin tissues were homogenized using a Polytron (Kinematica, Bohemia, ...



Innovative Research's Pooled Normal Human Serum was recently used in the following study:

(10-3-15)

Serum levels of phytoestrogens as biomarkers of intake in Mexican women

MR Robles-Burgeo, MI Ortega-Vlez - International Journal of..., 2015
... HPLC grade. Sodium acetate and formic acid were of reagent grade with a purity of 99% and 95%, respectively. Pooled human blank serum was purchased from Innovative Research (Novi, MI). Study subjects and study design. ...



Innovative Research's Single Donor Human Whole Blood was recently used in the following study:

(10-3-15)

Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification

CB Poole, L Ettwiller, NA Tanner, TC Evans Jr, S Wanji - PloS one, 2015
... Human whole blood was obtained from Innovative Research (Novi, MI). DNA quantity was determined using a Qubit dsDNA HS Assay kit in conjunction with a Qubit 2.0 Fluorometer as directed by the manufacturer.



Innovative Research's Human Prorenin 8x His was recently used in the following study:

(10-13-15)

Direct antiinflammatory effects of angiotensin(17) on microglia

M Liu, P Shi, C Sumners - Journal of Neurochemistry, 2015
... Human PRO was purchased from Innovative Research (Novi, MI). Immunocytochemistry Cultured microglial cells were washed with phosphate buffered saline (PBS; pH 7.2) and then fixed in 4% paraformaldehyde for 15 min at room temperature. ...



Innovative Research's Single Donor Human Whole Blood was recently used in the following study:

(10-13-15)

Total cobalt determination in human blood and synovial fluid using inductively coupled plasma-mass spectrometry: method validation and evaluation of performance

BD Kerger, R Gerads, H Gurleyuk, A Urban - & Environmental Chemistry, 2015
... Human tissue samples. The majority of the validation analyses were performed using single donor human whole blood or serum (Innovative Research, Novi, MI). Serum samples were also obtained from volunteers who participated ...



Innovative Research's Innovative Grade US Origin Sprague Dawley Rat Plasma was recently used in the following study:

(10-24-15)

PDF A Simple and Rapid LC-MS/MS Method for the Determination of BMCL26 a Novel Anti-Parasitic Agent in Rat Plasma

RR Voggu, X Zhou, B Su, B Guo - J Anal Bioanal Tech, 2015
... Waltham, Massachusetts, USA). Six individual lots of rat plasma (Sprague-Dawley rat plasmas K2) were obtained from Innovative Research (Novi, Michigan, USA) (Figure 1). Calibration standards and quality-control samples ...



Innovative Research's Pooled Normal Human Plasma was recently used in the following study:

(10-27-15)

Regulation of fibrin-mediated tumor cell adhesion to the endothelium using anti-thrombin aptamer

ER Gaddes, D Lee, G Gydush, Y Wang, C Dong - Experimental cell research, 2015
... Pooled human plasma was purchased from Innovative Research (Novi, MI) and all DNA sequences were obtained from Integrated DNA Technologies (Coralville, IA). The protein standard was purchased ...



Innovative Research's Pooled Normal Human AB Serum was recently used in the following study:

(10-29-15)

A pig-to-mouse coronary artery transplantation model for investigating the pathogenicity of anti-pig antibody

Y Lin, N Miyagi, GW Byrne, Z Du, H Kogelberg - Xenotransplantation, 2015
... recipient 20, 31. Naive human AB serum was commercially obtained (Innovative Research, Novi, MI). This serum was depleted of anti-pig antibody using three cycles of incubation with GTKO PAECs (1 10 7 cells/cycle). All serum ...

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Mitochondria

Starting in high school biology, were taught that mitochondria are the powerhouses of the cell. Further down the line, we learn that mutations or malfunctions within the powerhouse can cause a host of problems including disease states.

Researchers at Case Western Reserve University discovered a protein called Kruppel-like Factor 4 (KLF4) that is essential to boosting energy production within mitochondria. More importantly they noted that an absence of KLF4 reduced the production of energy, producing a phenotype that is extremely problematic for cardiac cells.

"Some cells are incredibly dependent on mitochondria, particularly the heart and brain," explained lead author Xudong Liao, Ph.D., an assistant professor of medicine at Case Western Reserve University School of Medicine, in the GEN article. "The brain is working all the time, too, even while we are sleeping, so it is particularly sensitive to mitochondrial function. Cancer also hijacks mitochondrial machinery to drive its spread. Therefore, the identification of KLF4 as a major regulator of mitochondrial health may have implications beyond those we detailed in this article."

KLFs regulate gene expression in many tissues. According to the article, KLF4 is involved in mitochondrial biogenesis, metabolic function, dynamics and autophagic clearance.

Looking at adult mice, researchers found that a deficiency of cardiac specific KLF4 had a potent effect on heart failure. They compared cardiac stress on normal mice and mice who had had the KLF4 gene knocked out. Nearly 50% of the knock out mice were overtaken by heart failure in a week and the rest had a stark decline in cardiac production.

Researchers hope that this study will provide the building blocks for further examination of KLF4 in tissues and possibly understand heart disease better.

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Congressional Funding

GEN released its 2015 Congressional Hall of Fame in June. The list, which features 10 representatives and 10 senators, focuses on members of Congress who have done the most to support federal spending for science research.

While the 2013 list included 30 representatives and 20 senators, the criteria for inclusion has been tightened. The guidelines were revised to be as follows: "10 representatives recognized at least five times for their support of federal spending for research in biopharma and/or all sciences, and 10 senators similarly recognized at least six times."

The compilation of awards and honors was put together through online searches of the members' biographies alongside those of announcements on organizations' web pages and of the organizations' records of past winners.

Michigan's own Fred Upton is on the list of representatives.

In alphabetical order, the list of representatives and senators is as follows:


-Rep. Michael C. Burgess, M.D. (R-TX)
-Rep. Diana DeGette (D-CO)
-Rep. Rosa DeLauro (D-CT)
-Rep. Anna Eshoo (D-CA)
-Rep. Chaka Fattah (D-MD)
-Rep. Cathy McMorris Rodgers (R-WA)
-Rep. David Price (D-NC)
-Rep. Debbie Wasserman Schultz (D-FL)
-Rep. Fred Upton (R-MI)
-Rep. Chris Van Hollen (D-MD)


-Sen. Sherrod Brown (D-OH)
-Sen. Richard Burr (R-NC)
-Sen. Bob Casey (D-PA)
-Sen. Susan Collins (R-ME)
-Sen. Richard J. Durbin (D-IL)
-Sen. Orrin Hatch (R-UT)
-Sen. Edward Markey (D-MA)
-Sen. Barbara Mikulski (D-MD)
-Sen. Jerry Moran (R-KS)
-Sen. Richard Shelby (R-AL)

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July References

(July 9, 2015) Innovative Research's Innovative Grade US Origin Wistar Rat Whole Blood was recently used in the following study:

A fluorescence assay for measuring acetylcholinesterase activity in rat blood and a human neuroblastoma cell line (SH-SY5Y)

MF Santillo, Y Liu - Journal of Pharmacological and Toxicological Methods, 2015
... (Manassas, VA, USA). Pooled whole blood from Wistar rats (containing EDTA) was purchased from Innovative Research Inc. (Novi, MI, USA). Chlorpyrifos-oxon and paraoxon were purchased from Chem Service, Inc. (West Chester, PA, USA). ...



(July 9, 2015) Innovative Research's Innovative Grade US Origin Sheep Serum was recently used in the following study:

Host Specificity of Ovine Bordetella parapertussis and the Role of Complement

SE Hester, LL Goodfield, J Park, HA Feaga, YV Ivanov - PloS one, 2015
... Briefly, blood collected from C57BL/6 mice or C3 -/- mice, was pooled, incubated at room temperature for 15 minutes and centrifuged at 250 x g for 10 min. Sheep serum was obtained from Innovative Research (Novi, MI). Complement ...



(July 15, 2015) Innovative Research's Sheep Anti Rat & Mouse Renin IgG Fraction was recently used in the following study:

Ultrastructural and Immunohistochemical Localization of the Plasma Membrane Ca2-ATPase 4 (PMCA4) in Ca2-Transporting Epithelia

RT Alexander, MR Beggs, R Zamani, N Marcussen - American Journal of , 2015
... antibodies against endothelial nitric oxide synthase (eNOS, R&D systems, Abingdon, United Kingdom). 7) 173 Sheep polyclonal anti-body recognizing mouse and rat renin (ISASRREN-GF, Innovative Research, Novi, 174 MI, USA). 175 176...



(July 20, 2015) Innovative Research's Pooled Normal Human Serum was recently used in the following study:

Enhanced immunogenicity of a respiratory syncytial virus (RSV) F subunit vaccine formulated with the adjuvant GLA-SE in cynomolgus macaques

K Patton, S Aslam, C Shambaugh, R Lin, D Heeke - Vaccine, 2015
... Test samples and positive control sera (IPLA-SER, Innovative Research, Novi MI) were heat inactivated, initially diluted 1:10, and 3-fold serially diluted in virus growth medium (VGM), comprised of minimum essential medium containing Earle's balance salt solution, 1 non ...



(July 21, 2015) Innovative Research's Chicken Red Blood Cells, Packed 5% was recently used in the following study:

Production of influenza virus-like particles from stably transfected Trichoplusia ni BT1 TN-5B1-4 cells

GP Hong, JH Park, HH Lee, KO Jang, DK Chung - and Bioprocess Engineering, 2015
...a series of 2-fold dilutions of VLPs (50 L, pelleted eluates of sucrose cushion ultracentrifu- gation and fractions from sucrose gradient ultracentrifu- gation) in PBS (pH 7.2) were mixed with 25 L of 1% (v/v) chicken red blood cells (Innovative Research, Novi, MI, USA) and ...



(July 22, 2015) Innovative Research's Innovative Grade US Origin Canine Plasma was recently used in the following study:

Sitagliptin Results in a Decrease of Truncated Apolipoprotein C1

NEB Skinner, MS Wroblewski, JA Kirihara - Diabetes Therapy
...Five healthy volunteers (2F/3 M, age 31.2 ? 8.6, BMI 23.2 ? 3.1) received 3-day administration of sitagliptin (100mg/day). Blood was drawn into citrate anticoagulant at baseline and 3 days. Mouse and dog plasmas were purchased from Innovative Research, Novi, MI, USA.



(July 22, 2015)Innovative Research's Innovative Grade US Origin Balb C Mouse Plasma was recently used in the following study:

Sitagliptin Results in a Decrease of Truncated Apolipoprotein C1

NEB Skinner, MS Wroblewski, JA Kirihara - Diabetes Therapy
...Five healthy volunteers (2F/3 M, age 31.2 ? 8.6, BMI 23.2 ? 3.1) received 3-day administration of sitagliptin (100mg/day). Blood was drawn into citrate anticoagulant at baseline and 3 days. Mouse and dog plasmas were purchased from Innovative Research, Novi, MI, USA.



(July 22, 2015) Innovative Research's Sheep Red Blood Cells, Packed 100% was recently used in the following study:

How do duration, frequency, and intensity of exogenous CORT elevation affect immune outcomes of stress?

GL McCormick, K Shea, T Langkilde - General and Comparative Endocrinology, 2015
... We washed SRBC (Innovative Research, Novi, MI) with phosphate buffered saline (PBS) and brought it to a 2% solution with PBS. We thawed 25l of plasma, diluted it 1:1 with PBS (25 L), and serially diluted this solution to 1:64 in a 96-well plate using a multichannel pipette.

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