{"product_id":"sars-cov-2-helicase-recombinant-protein-c-terminal-6his-tag-50ug","title":"SARS-CoV-2 Helicase Recombinant Protein C-terminal 6His Tag","description":"\u003cp\u003eSARS-CoV-2 Helicase Recombinant Protein C-terminal 6His Tag from Innovative Research has been recombinantly produced in E. coli. This is a Liquid protein buffered in Supplied as a 0.2 um filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5. with a purity of Greater than 80% as determined by reducing SDS-PAGE..\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eMore Details:\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eSpecies:\u003c\/b\u003e SARS-CoV-2 \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eTarget:\u003c\/b\u003e ORF1ab\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003ePurity:\u003c\/b\u003e Greater than 80% as determined by reducing SDS-PAGE. \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSource:\u003c\/b\u003e E. coli \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions:\u003c\/b\u003e Store below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. \u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003eThe non—structural protein 13 (nsp13) of SARS—CoV 2 is a helicase that separates double—stranded RNA or DNA with a 5'—3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5’-3’ direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom’s syndrome, Werner’s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.\u003cp\u003eThis recombinant protein can be used for biological assays. For research use only. . At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results.\u003c\/p\u003e","brand":"Innovative Research","offers":[{"title":"50UG","offer_id":40681335914692,"sku":"ICOV2HLCRCT6HIS50UG","price":808.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/products\/Enzymes-Proteins_ba39c02b-1c25-4368-9478-e9aac949ecc7.png?v=1632494460","url":"https:\/\/www.innov-research.com\/products\/sars-cov-2-helicase-recombinant-protein-c-terminal-6his-tag-50ug","provider":"Innovative Research","version":"1.0","type":"link"}