{"product_id":"rabbit-anti-smad7-polyclonal-antigen-affinity-purified-271","title":"Rabbit Anti SMAD7 Polyclonal Antigen affinity Purified (PBS with 0.05% NaN3 and 40% Glycerol, pH7.4) (Western Blot,IHC,ELISA)","description":"\u003cp\u003eRabbit Anti SMAD7 Polyclonal Antigen affinity Purified (PBS with 0.05% NaN3 and 40% Glycerol, pH7.4) (Western Blot,IHC,Sandwich ELISA) from Innovative Research is a polyclonal antibody in a liquid format, buffered in PBS with 0.05% NaN3 and 40% Glycerol, pH7.4 This antibody has been antigen affinity purified.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eMore Details:\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eHost:\u003c\/b\u003e Rabbit\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSpecies:\u003c\/b\u003e Human, Mouse, Rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eTarget:\u003c\/b\u003e SMAD7\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eClonality:\u003c\/b\u003e Polyclonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003ePurity:\u003c\/b\u003e Antigen affinity Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eImmunogen:\u003c\/b\u003e Synthetic peptide of human SMAD7\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions:\u003c\/b\u003e Store at -20°C. Avoid freeze \/ thaw cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eSMAD7,also named as Mothers against decapentaplegic homolog 7,is a 426 amino acid protein,which belongs to the dwarfin\/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1,which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.\u003c\/p\u003e\u003cp\u003eThis product can be used in analytical tools such as Western Blot,IHC,Sandwich ELISA.\u003c\/p\u003e\u003cp\u003eAt Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.\u003c\/p\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"120ul","offer_id":41694784815300,"sku":"IRBAMLSMAD7AAP271120UL","price":275.0,"currency_code":"USD","in_stock":true},{"title":"200ul","offer_id":41694784848068,"sku":"IRBAMLSMAD7AAP271200UL","price":427.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/f57f0ce8-b3b6-4e8b-b35d-92cb2b78a992_78d9100a-fac7-430a-aa0d-655815e443a1.jpg?v=1739998095","url":"https:\/\/www.innov-research.com\/products\/rabbit-anti-smad7-polyclonal-antigen-affinity-purified-271","provider":"Innovative Research","version":"1.0","type":"link"}