{"product_id":"mouse-phospholipase-a2-calcium-independent-elisa-kit","title":"Mouse Phospholipase A2 Calcium Independent ELISA Kit","description":"Mouse Phospholipase A2 Calcium Independent Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Phospholipase A2, Calcium Independent in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . iPLA2 present in samples reacts with an anti-Mouse iPLA2 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-iPLA2 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active iPLA2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse iPLA2. Detection Target: Mouse Phospholipase A2, Calcium Independent Reactivity: Mouse Range: 0.312-20ng\/mL Sensitivity: 0.09ng\/mL Storage Conditions: Store all components at 4┬░C This Mouse iPLA2 activity assay is for the quantitative determination of Phospholipase A2, Calcium Independent in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. \u003cp\u003e\n\u003cbr\u003e\n\u003c\/p\u003e\u003cp\u003e\n\u003cbr\u003e\n\u003c\/p\u003e\u003cul style=\"list-style-type: circle;\"\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003ePreheat a water bath to 37 C. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003ePlace the frozen plasma sample directly in the water bath. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003eGently agitate the plasma sample every 5-10 minutes to insure even thawing. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003eThe total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003eThe thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003ePlasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003ePlasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003eFrozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods. \u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp style=\"margin: 14px 0px;\"\u003e\u003cspan style=\"font-size:11.0pt;\"\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp style=\"margin: 14px 0px;\"\u003e\u003cspan style=\"font-size: 11pt; color: rgb(0, 0, 0);\"\u003eThis protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\n\u003cbr\u003e\n\u003c\/p\u003e \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":41712307470532,"sku":"IMSIPLA2KT","price":725.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_39e50793-5e8f-41fd-a1ec-797b2ecbba34.jpg?v=1739982353","url":"https:\/\/www.innov-research.com\/products\/mouse-phospholipase-a2-calcium-independent-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}