{"product_id":"mouse-interleukin-2-receptor-alpha-chain-cd25-elisa-kit","title":"Mouse Interleukin-2 Receptor Alpha Chain (CD25) ELISA Kit","description":"\u003cp\u003e Mouse Interleukin-2 Receptor Alpha Chain (CD25) Sandwich ELISA Kit is for quantitative detection of mouse CD25 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. \u003c\/p\u003e\n\u003cp\u003e The mouse CD25 Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD25 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-BiotinPeroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD25 amount of sample captured in plate.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003ci\u003eMore Details:\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eDetection Target:\u003c\/b\u003e Natural and recombinant Mouse CD25\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eReactivity:\u003c\/b\u003e Mouse\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRange:\u003c\/b\u003e 78pg\/ml-5000pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSensitivity:\u003c\/b\u003e \u0026lt; 5pg\/ml\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.\u003c\/p\u003e\n\u003cp\u003ePlease note – this product replaces our old product number IRKTAH5323 (same product, new number).\u003c\/p\u003e \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":28252536995920,"sku":"IMSIL2RAKT","price":744.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_7306aa08-7fc9-489b-b465-2ddb9160a87e.jpg?v=1739976819","url":"https:\/\/www.innov-research.com\/products\/mouse-interleukin-2-receptor-alpha-chain-cd25-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}