{"product_id":"mouse-endothelial-cell-specific-molecule-1-endocan-elisa-kit","title":"Mouse Endthelial Cell Specific Molecule-1 (Endocan) ELISA Kit","description":"\u003cp\u003e Mouse Endthelial Cell Specific Molecule-1 (Endocan) Sandwich ELISA Kit is for quantitative detection of mouse ESM1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. \u003c\/p\u003e\n\u003cp\u003e The mouse ESM1 Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ESM1 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ESM1 amount of sample captured in plate.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003ci\u003eMore Details:\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eDetection Target:\u003c\/b\u003e Natural and recombinant Mouse ESM1\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eReactivity:\u003c\/b\u003e Mouse\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRange:\u003c\/b\u003e 23.4pg\/ml-1500pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSensitivity:\u003c\/b\u003e \u0026lt; 10pg\/ml\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.\u003c\/p\u003e\n\u003cp\u003ePlease note – this product replaces our old product number IRKTAH5355 (same product, new number).\u003c\/p\u003e \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":28252537094224,"sku":"IMSESM1KT","price":744.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_d69b749d-64f0-4cec-9d50-81094f87acc6.jpg?v=1739976822","url":"https:\/\/www.innov-research.com\/products\/mouse-endothelial-cell-specific-molecule-1-endocan-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}