{"product_id":"innovative-grade-us-origin-rat-sprague-dawley-brain-tissue-lysate","title":"*Innovative Grade US Origin Rat Sprague Dawley Brain Tissue Lysate","description":"Select this product for Molecular Innovations name, Rat Brain Tissue Lysate and product number, RT-TL-BRInnovative Grade US Origin Rat Sprague Dawley Brain Tissue Lysate from Innovative Research flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C.More Details: Species: Sprague Dawley Rat Buffer: 0.1M Tris-HCl; 0.15M NaCl; 0.1% SDS; 0.5% Na Deoxycholate; 1% Triton X-100; pH 7.4 Source: Rat Brain Format: Frozen Liquid Storage Conditions: Store at -70░C for up to 5 years Shipping: Dry Ice \u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003eEach lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.Optimal experimental conditions and working dilutions must be determined by the end user. For research use only. At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results.Select this product for Molecular Innovations name, Rat Brain Tissue Lysate and product number, RT-TL-BR","brand":"Innovative Research","offers":[{"title":"200UG","offer_id":40748363481284,"sku":"IRTSDBRTL200UG","price":111.0,"currency_code":"USD","in_stock":true},{"title":"400UG","offer_id":40748363514052,"sku":"IRTSDBRTL400UG","price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/animal-biological-prod-thumb_8e6b21c1-3862-42e1-9842-1788ee87d3b9.jpg?v=1741281358","url":"https:\/\/www.innov-research.com\/products\/innovative-grade-us-origin-rat-sprague-dawley-brain-tissue-lysate","provider":"Innovative Research","version":"1.0","type":"link"}