{"product_id":"id688-mouse-tumor-necrosis-factor-receptor-superfamily-member-12a-cd266-tweakr-elisa-kit","title":"Mouse Tumor Necrosis Factor Receptor Superfamily Member 12A (CD266\/TweakR) ELISA Kit","description":"\u003cp\u003e This Mouse Tumor Necrosis Factor Receptor Superfamily Member 12A (CD266\/TweakR) Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of mouse TNFRSF12A\/TWEAKR in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests. \u003c\/p\u003e\n\u003cp\u003eThis mouse TNFRSF12A\/TWEAKR Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF12A\/TWEAKR has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: E.coli, E28-P801) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF12A\/TWEAKR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eDetection Target: \u003c\/b\u003eTumor Necrosis Factor Receptor Superfamily Member 12A (CD266\/TweakR) \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eUniprot ID: \u003c\/b\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q9CR75\" target=\"_blank\"\u003eQ9CR75\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eReactivity: \u003c\/b\u003eMouse\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eCross-Reactivity: \u003c\/b\u003eThere is no detectable cross-reactivity with other relevant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRange: \u003c\/b\u003e156pg\/ml-10000pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSensitivity: \u003c\/b\u003e\u0026lt;50pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions: \u003c\/b\u003eStore at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003e The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 17; 17 A3.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK\/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.; Immunogen sequence: 369 \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":31826754568272,"sku":"IMSTNFRSF102AKT","price":744.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.innov-research.com\/products\/id688-mouse-tumor-necrosis-factor-receptor-superfamily-member-12a-cd266-tweakr-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}