Mouse Hepatocyte Growth Factor Activator Inhibitor Type 1 (Kunitz-Type) ELISA Kit

Mouse Hepatocyte Growth Factor Activator Inhibitor Type 1 (Kunitz-Type) ELISA Kit


Innovative Research

  • $702.00

This Mouse Hepatocyte Growth Factor Activator Inhibitor Type 1 (Kunitz-Type) ELISA Kit from Innovative Research is intended for quantitative detection of mouse SPINT1 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.

This mouse SPINT1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SPINT1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E30-L507) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPINT1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SPINT1 amount of sample captured in plate.

  • Detection Target: Hepatocyte Growth Factor Activator Inhibitor Type 1 (Kunitz-Type)
  • Uniprot ID: Q9R097)
  • Reactivity: Mouse
  • Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
  • Range: 62.5pg/ml-4000pg/ml
  • Sensitivity: <12pg/ml
  • Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.; Immunogen sequence: 369

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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