{"product_id":"id688-mouse-contactin-4-elisa-kit","title":"Mouse Contactin 4 ELISA Kit","description":"\u003cp\u003e This Mouse Contactin 4 Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of mouse Contactin-4 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests. \u003c\/p\u003e\n\u003cp\u003eThis mouse Contactin-4 Sandwich ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Contactin-4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: D19-S1000) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Contactin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Contactin-4 amount of sample captured in plate.\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eDetection Target: \u003c\/b\u003eContactin 4 \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eUniprot ID: \u003c\/b\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q69Z26\" target=\"_blank\"\u003eQ69Z26\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eReactivity: \u003c\/b\u003eMouse\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eCross-Reactivity: \u003c\/b\u003eThere is no detectable cross-reactivity with other relevant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRange: \u003c\/b\u003e312pg\/ml-20000pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSensitivity: \u003c\/b\u003e\u0026lt;50pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions: \u003c\/b\u003eStore at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003e The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 6; 6 E1. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.; Immunogen sequence: 369 \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":31766917808208,"sku":"IMSCNTN4KT","price":943.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_d6ef46a9-97cc-4e39-90a2-600159f24787.jpg?v=1739977811","url":"https:\/\/www.innov-research.com\/products\/id688-mouse-contactin-4-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}