{"product_id":"id688-human-metalloproteinase-inhibitor-2-elisa-kit","title":"Human Metalloproteinase Inhibitor 2 ELISA Kit","description":"\u003cp\u003e This Human Metalloproteinase Inhibitor 2 Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of human TIMP-2 in cell culture supernates, serum, plasma (heparin , EDTA) and saliva. Strip well format. Reagents for up to 96 tests. \u003c\/p\u003e\n\u003cp\u003eThis human TIMP-2 Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-2 has been precoated onto 96-well plates. Standards(CHO, C27-P220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-2 amount of sample captured in plate.\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eDetection Target: \u003c\/b\u003eMetalloproteinase Inhibitor 2 \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eUniprot ID: \u003c\/b\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P16035\" target=\"_blank\"\u003eP16035\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eReactivity: \u003c\/b\u003eHuman\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eCross-Reactivity: \u003c\/b\u003eThere is no detectable cross-reactivity with other relevant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRange: \u003c\/b\u003e156pg\/ml-10000pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSensitivity: \u003c\/b\u003e\u0026lt;2pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions: \u003c\/b\u003eStore at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003e The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs(kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.; Immunogen sequence: 380 \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":31685020975184,"sku":"IHUTIMP2KT","price":756.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_570d5c09-3186-4551-b689-0fa7b05ca9ea.jpg?v=1739977647","url":"https:\/\/www.innov-research.com\/products\/id688-human-metalloproteinase-inhibitor-2-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}