{"product_id":"id688-human-epidermal-growth-factor-receptor-elisa-kit","title":"Human Epidermal Growth Factor Receptor ELISA Kit","description":"\u003cp\u003e This Human Epidermal Growth Factor Receptor Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of human EGFR in cell culture supernates, serum, plasma (heparin, EDTA) and human milk. Strip well format. Reagents for up to 96 tests. \u003c\/p\u003e\n\u003cp\u003eThis human EGFR Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGFR has been precoated onto 96-well plates. Standards(NSO, M1-S645) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGFR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EGFR amount of sample captured in plate.\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eDetection Target: \u003c\/b\u003eEpidermal Growth Factor Receptor \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eUniprot ID: \u003c\/b\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P00533\" target=\"_blank\"\u003eP00533\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eReactivity: \u003c\/b\u003eHuman\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eCross-Reactivity: \u003c\/b\u003eThere is no detectable cross-reactivity with other relevant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRange: \u003c\/b\u003e156pg\/ml-10000pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSensitivity: \u003c\/b\u003e\u0026lt;1pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions: \u003c\/b\u003eStore at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003e The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: The epidermal growth factor receptor(EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family(EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR(ErbB-1), HER2\/c-neu(ErbB-2), Her 3(ErbB-3) and Her 4(ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha(TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression(known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.; Immunogen sequence: 320 \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":31281799888976,"sku":"IHUEGFRKT","price":744.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_a540a614-86af-4fe8-b6d0-4ddec9d1dc51.jpg?v=1739977241","url":"https:\/\/www.innov-research.com\/products\/id688-human-epidermal-growth-factor-receptor-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}