{"product_id":"id688-canine-bone-morphogenetic-protein-5-high-sensitivity-elisa-kit","title":"Canine Bone Morphogenetic Protein 5 High-Sensitivity ELISA Kit","description":"\u003cp\u003e This Canine Bone Morphogenetic Protein 5 High-Sensitivity Sandwich ELISA Kit from Innovative Research is intended is a high-sensitivity sandwich Sandwich ELISA kit for quantitative detection of Dog Caninea BMP-5. 96wells\/kit, with removable strips. Strip well format. Reagents for up to 96 tests. \u003c\/p\u003e\n\u003cp\u003eThis Canine BMP-5 Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-5 has been precoated onto 96-well plates. Standards(NSO, A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Canine BMP-5 amount of sample captured in plate.\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eDetection Target: \u003c\/b\u003eBone Morphogenetic Protein 5 \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eUniprot ID: \u003c\/b\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P22003\" target=\"_blank\"\u003eP22003\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eReactivity: \u003c\/b\u003eCanine\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eCross-Reactivity: \u003c\/b\u003eThere is no detectable cross-reactivity with other relevant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRange: \u003c\/b\u003e156pg\/ml-10,000pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSensitivity: \u003c\/b\u003e\u0026lt;10pg\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions: \u003c\/b\u003eStore at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003e The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: Bone morphogenetic protein 5 is a protein that in Dog Canineas is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on Dog Caninea chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.; Immunogen sequence: 400 \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":31965416489040,"sku":"ICNBMP5KTH","price":773.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_9d4b696a-a7a4-4efb-acaf-5d56001a85a4.jpg?v=1739978399","url":"https:\/\/www.innov-research.com\/products\/id688-canine-bone-morphogenetic-protein-5-high-sensitivity-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}