{"product_id":"human-topoisomerase-ii-elisa-kit","title":"Human Topoisomerase II ELISA Kit","description":"Human Topoisomerase II Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Human Topoisomerase II in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . TOP2 present in samples reacts with an anti-Human TOP2 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-TOP2 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active TOP2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human TOP2. Detection Target: Human Topoisomerase II Reactivity: Human Range: 0.625-40ng\/mL Sensitivity: 0.225ng\/mL Storage Conditions: Store all components at 4┬░C This Human TOP2 activity assay is for the quantitative determination of Topoisomerase II in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":41712449781956,"sku":"IHUTOP2KT","price":707.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_184f8db5-0355-4d96-9180-2093897307ff.jpg?v=1739985046","url":"https:\/\/www.innov-research.com\/products\/human-topoisomerase-ii-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}