{"product_id":"human-plasma-kallikrein-recombinant-protein-c-6-his-tag-50ug","title":"Human Plasma kallikrein Recombinant Protein C-6 His Tag","description":"Human Plasma kallikrein Recombinant Protein C-6 His Tag from Innovative Research has been recombinantly produced in Human Cells. This is a Liquid protein buffered in Supplied as a 0.2 um filtered solution of 20mM TrisHCl,150mM NaCl,10% Glycerol,pH8.0. It is not recommended to reconstitute to a concentration less than 100UG\/ml. with a purity of Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng\/ug (1 IEU\/ug) as determined by LAL test..More Details: Species: Human Target: KLKB1 Purity: Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng\/ug (1 IEU\/ug) as determined by LAL test. Source: Human Cells Storage Conditions: Store at -20░C, stable for 6 months after receipt.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003eKLKB1 is a 638 amino acids protein that belongs to the peptidase S1 family and plasma kallikrein subfamily. It contains a peptidase S1 domain and four apple domains. The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. It participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation.Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.This recombinant protein can be used for biological assays. For research use only. . At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results. \u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cdiv style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; color: rgb(69, 69, 69); font-family: Inter, sans-serif; font-size: 16px; text-align: left;\" id=\"isPasted\"\u003eCurious About The Best Way To Thaw Plasma?\u003c\/div\u003e\u003cdiv style='user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; color: rgb(11, 9, 16); font-family: InterVariable, sans-serif, \"Apple Color Emoji\", \"Segoe UI Emoji\", \"Segoe UI Symbol\", \"Noto Color Emoji\"; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;'\u003e\u003cbr style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat;\"\u003e\u003c\/div\u003e\u003cdiv style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; color: rgb(69, 69, 69); font-family: Inter, sans-serif; font-size: 16px; text-align: left;\"\u003e\n\u003ca href=\"https:\/\/www.innov-research.com\/blogs\/news\/updated-video-resource-how-to-properly-thaw-plasma-samples\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eCheck out this\u003c\/a\u003e\u003cspan style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat;\"\u003e\u003ca href=\"https:\/\/www.innov-research.com\/blogs\/news\/updated-video-resource-how-to-properly-thaw-plasma-samples\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e \u003c\/a\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/www.innov-research.com\/blogs\/news\/updated-video-resource-how-to-properly-thaw-plasma-samples\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eblog post\u003c\/a\u003e\n\u003c\/div\u003e\u003cdiv style='user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; color: rgb(11, 9, 16); font-family: InterVariable, sans-serif, \"Apple Color Emoji\", \"Segoe UI Emoji\", \"Segoe UI Symbol\", \"Noto Color Emoji\"; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;'\u003e\u003cbr style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat;\"\u003e\u003c\/div\u003e\u003cul style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; color: rgb(11, 9, 16); font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; list-style-position: inside; padding-left: 2rem; font-family: Inter, sans-serif; text-align: left; list-style-type: circle;\"\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit;\"\u003ePreheat a water bath to 37 C.\u003c\/li\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit;\"\u003ePlace the frozen plasma sample directly in the water bath.\u003c\/li\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit;\"\u003eGently agitate the plasma sample every 5-10 minutes to insure even thawing.\u003c\/li\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit;\"\u003eThe total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.\u003c\/li\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit;\"\u003eThe thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.\u003c\/li\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit;\"\u003ePlasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.\u003c\/li\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit;\"\u003ePlasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.\u003c\/li\u003e\n\u003cli style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; list-style: inherit; margin-bottom: 0px;\"\u003eFrozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cdiv style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; color: rgb(11, 9, 16); font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; font-family: Inter, sans-serif; text-align: left;\"\u003e \u003c\/div\u003e\u003cdiv style='user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; color: rgb(11, 9, 16); font-family: InterVariable, sans-serif, \"Apple Color Emoji\", \"Segoe UI Emoji\", \"Segoe UI Symbol\", \"Noto Color Emoji\"; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;'\u003e\u003cbr style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat;\"\u003e\u003c\/div\u003e\u003cdiv style=\"user-select: initial; cursor: inherit; box-sizing: content-box; background-repeat: no-repeat; color: rgb(11, 9, 16); font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; font-family: Inter, sans-serif; text-align: left;\"\u003eThis protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results\u003c\/div\u003e","brand":"Innovative Research","offers":[{"title":"50UG","offer_id":40681288728772,"sku":"IHUPLAKLKRC6HIS50UG","price":808.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/products\/Enzymes-Proteins_3250242e-4d84-4469-a773-556659a2de47.png?v=1632494133","url":"https:\/\/www.innov-research.com\/products\/human-plasma-kallikrein-recombinant-protein-c-6-his-tag-50ug","provider":"Innovative Research","version":"1.0","type":"link"}