{"product_id":"human-arachidonate-5-lipoxygenase-elisa-kit","title":"Human Arachidonate-5-Lipoxygenase ELISA Kit","description":"Human Arachidonate-5-Lipoxygenase Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Human Arachidonate-5-Lipoxygenase in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . ALOX5 present in samples reacts with an anti-Human ALOX5 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-ALOX5 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active ALOX5 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human ALOX5. Detection Target: Human Arachidonate-5-Lipoxygenase Reactivity: Human Range: 0.156-10ng\/mL Sensitivity: 0.057ng\/mL Storage Conditions: Store all components at 4┬░C This Human ALOX5 activity assay is for the quantitative determination of Arachidonate-5-Lipoxygenase in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. \u003cbr\u003e\u003ca href=\"https:\/\/youtu.be\/J7fuMk6JfyI?si=cvhwIRlxjJlTCI3m\"\u003eClick here\u003c\/a\u003e to view a video tutorial covering frequently asked questions regarding ELISA workflows.","brand":"Innovative Research","offers":[{"title":"Default Title","offer_id":41712129540292,"sku":"IHUALOX5KT","price":742.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/files\/8044494f-a574-4da9-a905-b780d80c69bf_41a95657-1e5f-4bc4-9138-2f27f5dce400.jpg?v=1739979471","url":"https:\/\/www.innov-research.com\/products\/human-arachidonate-5-lipoxygenase-elisa-kit","provider":"Innovative Research","version":"1.0","type":"link"}