{"product_id":"bacteria-derived-streptococcus-pyogenes-ides-recombinant-protein-his-tag","title":"Bacteria-Derived Streptococcus pyogenes IdeS Recombinant Protein His Tag","description":"\u003cp\u003eBacteria-Derived Streptococcus pyogenes IdeS Recombinant Protein His Tag from Innovative Research has been recombinantly produced in E. coli. This is a Liquid protein buffered in PBS, pH7.4 with a purity of \u0026gt;95% as determined by SDS-PAGE..\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eMore Details:\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eSpecies:\u003c\/b\u003e Bacteria-Derived \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eTarget:\u003c\/b\u003e \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003ePurity:\u003c\/b\u003e \u0026gt;95% as determined by SDS-PAGE. \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eSource:\u003c\/b\u003e E. coli \u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eStorage Conditions:\u003c\/b\u003e Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. \u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAdditional Information:\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003eIdeS from Streptococcus pyogenes, also known as Mac-1, is an endopeptidase with specificity for IgG. The bacterial protein Mac-1 adopts an alpha\/beta fold, with 14 beta strands and 9 alpha helices. The N-terminal domain is made up predominantly of alpha helices, whereas the C-terminal domain consists predominantly of beta sheets. Mac 1 blocks polymorphonuclear opsonophagocytosis, inhibits the production of reactive oxygen species and contains IgG endopeptidase activity.IdeS is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab‘)2 and Fc\/2 fragments. IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species. IdeS is cloned from Streptococcus pyogenes and expressed in E. coli. \u003cp\u003eThis protein carries a polyhistidine Tag at the N-terminal. The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).. At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results.\u003c\/p\u003e","brand":"Innovative Research","offers":[{"title":"2500U","offer_id":40245426815172,"sku":"IBCTSTPCPYGNIDESRHIS2500U","price":678.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0050\/2297\/4065\/products\/Enzymes-Proteins_41975e1b-45be-4403-aa99-eeff19d1970f.png?v=1627670439","url":"https:\/\/www.innov-research.com\/products\/bacteria-derived-streptococcus-pyogenes-ides-recombinant-protein-his-tag","provider":"Innovative Research","version":"1.0","type":"link"}