Rat uPA Assay and ELISA Kits

Rat uPA Total ELISA Kit

Catalog No.: IRUPAKT-TOT


Category: Assay and ELISA Kits

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Product Description

Rat uPA Total ELISA Kit

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration.

Rat uPA total antigen assay is intended for the quantitative determination of total urokinase plasminogen activator in rat plasma, serum, urine, cell culture media, or tissue extracts. The assay measures uPA antigen in the 0.025 - 10 ng/ml range. If the unknown is thought to have high uPA levels, dilutions may be made in blocking buffer or similar biological fluid devoid of uPA. Strip well format. Reagents for up to 96 tests.

Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.

Detection Target: Urokinase
Reactivity: Rat
Range: 0.025 - 10 ng/ml
Sensitivity: 0.01 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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Rat uPA Total ELISA Kit

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