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Mouse tPA Assay and ELISA Kits

Mouse tPA Total ELISA Kit

Catalog No.: IMTPAKT-TOT

$495

Category: Assay and ELISA Kits

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Product Description

Mouse tPA Total ELISA Kit


tPA is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases.

This mouse tissue-type plasminogen activator (tPA) total antigen assay is intended for the quantitative determination of total tPA in mouse plasma and other biological fluids. The assay measures tPA antigen in the 0.1 - 50 ng/ml range. If the unknown is thought to have high tPA levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.

Mouse tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free, latent and complexed tPA will bind to the plate. After appropriate washing steps, monoclonal anti-mouse tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse tPA. Color development is proportional to the concentration of tPA in the samples.

Detection Target: TPA
Reactivity: Mouse
Range: 0.1 - 50 ng/ml
Sensitivity: 0.035 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
(12-30-09)

Endothelial Progenitor Populations in Differentiating Embryonic Stem Cells. II. Drug Selection and Functional Characterization


S Kim, HA von Recum - Tissue Engineering Part A, 2009 - online.liebertpub.com
...section. Both Mouse tPA Activity Assay (cat. no. IMTPAKT; Innovative Research, Novi, MI) and Mouse tPA Total Antigen Assay (cat. no. IMTPAKT-TOT; Innovative Research) were used according to the manufacturer's protocol. tPA...

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Mouse tPA Total ELISA Kit

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