Mouse PAI-1 Total ELISA Kit
PAI-1 is a central regulator of the blood fibrinolytic system. Clinical studies have indicated that increased PAI-1 levels increase the risk for thrombosis, whereas decreased levels may cause recurrent bleeding.
This mouse plasminogen activator inhibitor type 1 (PAI- 1) total assay is intended for the quantitative determination of total PAI-1 in mouse plasma in citrate, EDTA or heparin, serum, cell culture media, tissue lysates and other biological fluids. The assay measures PAI-1 antigen in the 0.05 - 50 ng/ml range. If the unknown is thought to have high PAI 1 levels, dilutions may be made in plasma devoid of PAI 1 (cat IMPLA-SC-PAI) or in blocking buffer. A 1:5 dilution for normal plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Mouse PAI-1 will bind to the monoclonal capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. After appropriate washing steps, polyclonal anti-mouse PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse PAI-1. Color development is proportional to the concentration of PAI- 1 in the samples.
Detection Target: PAI-1
Range: 0.05 - 50 ng/ml
Sensitivity: 0.031 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
Changes in spontaneous thrombolytic activity during progression of atherosclerosis in Apo-/- and LDLR-/- double knockout miceTakumi Sato, Manami Yoshimura, Tomohide Sanda, Kanae Hyodo, Junichiro Yamamoto, Hiromitu Ishii, Tsutomu Yamashita
International Journal of Clinical and Experimental Pathology
"... After 14 and 22 weeks of feeding the high-fat diet, blood was drawn from the abdominal aorta of mice into 3.14% sodium citrate by using a 23-gauge needle. All samples (citrated plasma) were stored at -80C until analysis. Plasma PAI-1 levels were determined by using a commercial ELISA kit (IMPAIKT-TOT, Innovative Research, Novi, Michigan, USA).
Experimental and Therapeutic Medicine, August 2018
... PAI-1 and TGF-beta levels in BAL fluids were measured at 0, 4, 12, 18, and 24 weeks after irradiation using ELISA kits for PAI-1 (Innovative Research, Novi, MI, USA)... To determine the effect of thoracic irradiation on the expression of PAI-1 and TGF-beta in the lung, PAI-1 and TGF-beta levels in BAL fluid were measured using ELISA kits. In WT mice, PAI-1 levels in BAL fluids were unchanged from baseline until 12 weeks after irradiation but started increasing after 18 weeks and reached their highest levels at 24 weeks after irradiation..."(11-24-08)
S Loubele, CA Spek, P Leenders... - ... of Thrombosis and ..., 2009 - Wiley Online Library
...PAI-1 antigen. Total PAI-1 antigen levels were determined in tissue homogenates (1 mg mL -1 protein content) according to the manufacturer's instructions (Innovative Research, Novi , MI, USA). CD 45 staining. A method description is supplied online in the data supplements. (1-24-10)
T Senoo, N Hattori, T Tanimoto, M Furonaka... - Thorax, 2010 - thorax.bmj.com
...The levels of PAI-1 and the activity of functionally active uPA in BAL fluid were measured using a total murine PAI-1 antigen assay kit (Innovative Research, Novi, Michigan, USA) and a mouse uPA activity assay kit (Innovative Research), respectively, following the... (10-21-10)
PK Henke, M Mitsuya, CE Luke... - ... , and vascular biology, 2011 - Am Heart Assoc
...underwent general anesthesia with isoflorane/O 2 . All animal studies were done with University of Michigan Animal Use...thrombus homogenate was assessed for urokinase plasminogen activator and plasminogen activator inhibitor-1 by ELISA (Innovative Research, Novi, Mich). (5-1-11)
Hypoxia-inducible factor-1a mediates TGF--induced PAI-1 production in alveolar macrophages in pulmonary fibrosis
M Ueno, T Maeno, M Nomura... - American Journal of ..., 2011 - Am Physiological Soc
...ELISA. The concentration of PAI-1 protein in the BAL fluid and culture medium was measured using the mouse active PAI-1 ELISA kit (Innovative Research, Novi, MI) and PDGF-AA ELISA kit (R&D Systems, Minneapolis, MN), according to the manufacturer's instructions. (7-24-11)
Fibrinolysis is down-regulated in mouse collagen-induced arthritis, but its normalization does not alleviate the course of disease
J Kwiecinski, E Josefsson... - Inflammation Research, 2011 - Springer
...Plasma levels of PAI-1 were measured with Murine PAI-1 Total Antigen Assay (Innovative Research, Novi, MI, USA), plasma active tPA levels were measured with Mouse tPA Activity Elisa Assay (Innovative Research) and plasma levels of D-dimer were measured with... (12-1-11)
Intra-airway administration of small interfering RNA targeting plasminogen activator inhibitor-1 attenuates allergic asthma in mice
S Miyamoto, N Hattori, T Senoo... - American Journal of ..., 2011 - Am Physiological Soc
...PAI-1, TGF-, and hepatocyte growth factor (HGF) levels in samples were measured using ELISA kits for PAI-1 (Innovative Research, Novi, MI), for TGF- (R&D Systems, Minneapolis, MN), and for HGF (Institute of Immunology, Tokyo, Japan), according to the manufacturers... (3-22-14)
Resistin regulates the expression of plasminogen activator inhibitor-1 in 3T3-L1 adipocytes
Y Ikeda, H Tsuchiya, S Hama, K Kajimoto, K Kogure - and Biophysical Research, 2014
PAI-1 released from 3T3-L1 cells was measured using the Mouse PAI-1 Total ELISA Kit (Innovative Research Inc, Novi, MI, USA) according to the manufacturer's instructions. 2.5. Western blotting... (2-27-15)
Effect of ascorbate on plasminogen activator inhibitor-1 expression and release from platelets and endothelial cells in an in-vitro model of sepsis.
SB Swarbreck, D Secor, CG Ellis, MD Sharpe - Blood Coagulation & , 2015
... activator inhibitor-1 protein expression and release. Both intracellular PAI-1 protein (ie PAI-1 in cell lysates) and PAI-1 release (ie PAI-1 in cell supernatants) were quantified using the total PAI-1 ELISA kit (IMPAIKT- TOT; Innovative Research, Novi, Michigan, USA) according to...