Rhesus Monkey Factor VIII Total Antigen ELISA Kit
Factor VIII (aka Factor VIII:C or Antihemophilic Globulin) is a glycoprotein zymogen that circulates in a stabilized non-covalent complex with von Willebrand Factor (vWF). Following activation by thrombin or Factor Xa, Factor VIIIa dissociates from vWF and catalyzes the activation of Factor X by Factor IXa in the amplification phase of coagulation. Factor VIIIa activity is quickly decreased by spontaneous dissociation and proteolytic degradation by activated Protein C, Factor Xa and Factor IXa. Hemophilia A is caused by mutations in the Factor VIII gene; a majority of patients have decreased Factor VIII plasma levels while 5% of patients have normal levels of nonfunctioning protein.
This rhesus macaque monkey (Macaca mulatta) coagulation Factor VIII antigen assay is intended for the quantitative determination of total Factor VIII antigen in rhesus plasma. The assay measures Factor VIII antigen in the 0.001 - 0.48 IU/ml range. 1:8 and 1:16 dilutions for normal plasma, or 1:4 and 1:8 dilutions for Haemophiliac plasma, are suggested for best results. Strip well format. Reagents for up to 96 tests.
Rhesus Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rhesus Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rhesus plasma. Color development is proportional to the concentration of Factor VIII in the samples.
Detection Target: Factor VIII
Reactivity: Rhesus Monkey
Range: 0.001 - 0.48 IU/ml
Sensitivity: 0.00023 IU/ml
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