Rhesus Monkey Factor VIII Total Antigen ELISA Kit
This rhesus macaque monkey (Macaca mulatta) coagulation Factor VIII antigen assay is intended for the quantitative determination of total Factor VIII antigen in rhesus plasma. For research use only.
Rhesus Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rhesus Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rhesus plasma. Color development is proportional to the concentration of Factor VIII in the samples.