SPARC Osteonectin ELISA Kit (Rat / Mouse)
Rat / Mouse SPARC ELISA employs the quantitatively competitive enzyme immunoassay technique in which Rat / Mouse SPARC present in samples competed with a fixed amount of biotinylated Rat / Mouse SPARC for sites on purified rabbit IgG specific against Rat / Mouse SPARC. During the incubation, the standard and samples bound to the Anti SPARC IgG that are pre-coated onto the microplates. The biotinylated SPARC are competitively bound to antibody specific to SPARC. Following a wash to remove any unbound standard, samples and biotin conjugate, a Streptavidin conjugated to horseradish-peroxidase (HRP) is added to the wells. After washing away any unbound enzyme, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of Rat / Mouse SPARC bound in the initial step. The sample values are then read off the standard curve. Rat / Mouse SPARC ELISA has been shown to accurately quantify the recombinant and natural Rat / Mouse SPARC. Results obtained using natural Rat / Mouse SPARC showed dose response curves that were parallel to the standard curves obtained using the kit standards.