Rat uPA ELISA Kit
Urokinase plasminogen activator is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration.
Rat uPA activity assay is intended for the quantitative determination of active urokinase plasminogen activator in rat plasma. The assay measures uPA activity in the 0.02 - 10 ng/ml range. If the unknown is thought to have high uPA levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.
Functionally active uPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
Detection Target: Urokinase
Range: 0.02 - 10 ng/ml
Sensitivity: 0.019 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.