Rat Prorenin / Renin Total Antigen ELISA Kit
Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects.
This rat prorenin/renin total antigen assay is intended for the quantitative determination of total prorenin and renin antigen in rat plasma and serum. The assay measures prorenin and renin antigen in the 0.1 - 100 ng/ml range. If the unknown is thought to have high prorenin/renin levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.
Rat prorenin and renin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin-labeled polyclonal anti-rat prorenin antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prorenin. Color development is proportional to the total concentration of prorenin and renin in the samples.
Detection Target: Prorenin
Range: 0.1 - 100 ng/ml
Sensitivity: 0.062 ng/ml
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