Rat Prolactin ELISA Kit
Rat prolactin (PRL) is a 197 aa, 23kD peptide hormone that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk.
This rat prolactin antigen assay is intended for the quantitative determination of prolactin antigen in rat plasma. The assay measures total rat prolactin in the 2-1000 ng/mL range. Samples with rat prolactin levels above 1000ng/mL should be diluted in blocking buffer before use. Normal plasma should not require dilution before use in this assay. Strip well format. Reagents for up to 96 tests.
Rat prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, anti-rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prolactin. Color development is proportional to the concentration of prolactin in the samples.
Detection Target: Prolactin
Range: 2 - 1000 ng/ml
Sensitivity: 0.168 ng/ml
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