Rat PAI-1 Assay and ELISA Kits

Rat PAI-1 Total ELISA Kit

Catalog No.: IRPAIKT-TOT


Category: Assay and ELISA Kits

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Product Description

Rat PAI-1 Total ELISA Kit

Plasminogen activator inhibitor-1 (PAI-1) is a central regulator of the blood fibrinolytic system. Clinical studies have indicated that increased PAI-1 levels increase the risk for thrombosis, whereas decreased levels may cause recurrent bleeding.

This rat plasminogen activator inhibitor type 1 (PAI-1) total antigen assay is intended for the quantitative determination of total PAI-1 in biological fluids. The assay measures total PAI-1 in the 0.05 - 50 ng/ml range. A 1:2-1:10 dilution for normal rat plasma is suggested for best results. If the unknown is thought to have high PAI 1 levels, dilutions may be made in plasma devoid of PAI-1 (Cat IRPLA-SC-PAI) or in blocking buffer. Strip well format. Reagents for up to 96 tests.

Rat PAI-1 present in samples reacts with the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. After appropriate washing steps, biotin labeled primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat PAI-1. The amount of color development is directly proportional to the concentration of total PAI-1 in the sample.

Detection Target: PAI-1
Reactivity: Rat
Range: 0.05 - 50 ng/ml
Sensitivity: 0.022 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.


Inhibitory effect of water extractive components from heshiko and narezushi on plasma PAI-1 activity in rats

K ITO - , 2016
The content of the total PAI-1 in plasma was measured using a commer- cial kit (Total Rat PAI-1 ELISA kit; Innovative Research Inc., Novi, MI, USA according to the manufacturer's protocol....


Losartan reduces liver expression of plasminogen activator inhibitor-1 (PAI-1) in a high fat-induced rat nonalcoholic fatty liver disease model

MS Rosselli, AL Burgueo, J Carabelli, M Schuman... - Atherosclerosis, 2009 - Elsevier
...Total PAI-1 levels in liver were measured in duplicate using an enzyme-linked immunoassay specific for rat PAI-1 according to the manufacturer's instructions (Rat PAI-1 Total Antigen Assay, Innovative Research, Novi, MI, USA). The sensitivity of the assay is = 0.032 ng/mL.

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Rat PAI-1 Total ELISA Kit

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