Rat PAI-1 Assay and ELISA Kits


Catalog No.: IRPAIKT


Category: Assay and ELISA Kits

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Product Description


Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor that is an important regulator of fibrinolysis and extracellular matrix turnover. PAI 1 may be important in hepatocyte growth and proliferation in vivo. Increased PAI-1 levels may increase the risk for myocardial infarction, atherosclerosis, and retenosis. Increased PAI-1 levels may also play an important role of the development and pathogenesis of diabetic nephropathy. Decreased levels may reduce thrombotic events.

This rat plasminogen activator inhibitor type 1 (PAI-1) activity assay is intended for the quantitative determination of active PAI-1 in rat plasma and serum. The assay measures active PAI-1 in the 0.1 - 50 ng/ml range. Plasma and serum samples should be applied directly to the plate without dilution. Strip well format. Reagents for up to 96 tests.

Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. Unbound PAI-1 samples are washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.

Detection Target: PAI-1
Reactivity: Rat
Range: 0.1 - 50 ng/ml
Sensitivity: 0.032 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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