Rat GST Yb1 (mu) ELISA KitIn this assay, the GST Yb1 present in samples reacts with the anti-GST Yb1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-GST Yb1 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound GST Yb1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of GST Yb1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of GST Yb1 in the test sample. The quantity of GST Yb1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.