Rat Fibrinogen ELISA Kit
Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis.
This rat fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in rat plasma and serum. The assay measures total rat fibrinogen in the 3.125 - 800 ng/ml range. If the unknown is thought to have high fibrinogen levels, dilutions may be made in diluent. It is suggested that normal rat plasma samples be diluted 1:500,000 to 1:1,000,000 in diluent. Strip well format. Reagents for up to 96 tests.
Rat fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-rat fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.
Detection Target: Fibrinogen
Range: 3.125 - 800 ng/ml
Sensitivity: 0.184 ng/ml
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