Rat Ferritin Assay and ELISA Kits

Rat Ferritin ELISA Kit

Catalog No.: IRKTAH1179


Category: Assay and ELISA Kits

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Product Description

Rat Ferritin ELISA Kit

In this assay the ferritin present in samples reacts with the anti-ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of ferritin in the test sample. The quantity of ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Up-regulation of heme oxygenase-1 in rat spleen after aniline exposure

J Wang, H Ma, PJ Boor, VM Ramanujam... - Free Radical Biology ..., 2010 - Elsevier
...After incubating 30 min on ice, the extracts were centrifuged at 14,000 g for 30 min. The supernatant was saved and used for ferritin assay. Ferritin was quantitated using an ELISA kit, essentially as described by the manufacturer (Innovative Research, Novi, MI, USA).

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Rat Ferritin ELISA Kit

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