Rat C3 ELISA Kit
In this assay the C3 present in samples reacts with the anti-C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C3 in the test sample. The quantity of C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
The Journal of Immunology, April 2018
... To confirm the deficiency of the C3 protein in the C3 KO rats, C3 levels were measured in sera collected from both WT and KO rats using a commercially available rat C3 ELISA kit (Innovative Research, Novi, MI) following the manufacturer's protocol ...(3-15-14)
Integrative analysis reveals novel pathways mediating the interaction between adipose tissue and pancreatic islets in obesity in rats
R Malpique, H Figueiredo, Y Esteban, SA Rebuffat - Diabetologia, 2014
Validation studies were performed, according to the manufacturer's recommendations, through the following quantitative ELISA immunoassays: Rat Complement C3 (Innovative Research, Novi, MI, USA)...