Rat ANP ELISA Kit
The Rat ANP ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of rat ANP in plasma, serum, tissue extract, and cell culture supernatant samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rat ANP in less than 5 hours. A polyclonal antibody specific for rat ANP has been pre-coated onto a 96-well microplate with removable strips. The rat ANP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for ANP, which is recognized by streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.