Rat alpha Macroglobulin ELISA KitThe Rat a-Macroglobulin ELISA kit is designed for detection of rat a-macroglobulin in rat plasma, serum, urine, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures a-macroglobulin in less than 4 hours. A polyclonal antibody specific for a-macroglobulin has been pre-coated onto a microplate. Rat a-macroglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for a- macroglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.