Rabbit PAI-1 ELISA Kit
PAI-1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.
Rabbit PAI-1 activity assay is intended for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in rabbit plasma. The assay measures PAI-1 activity levels in the 0.05 - 50 ng/ml range. If the unknown is thought to have high PAI 1 activity levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.
Functionally active PAI-1 present in plasma will bind to the urokinase (uPA) coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, anti-human PAI-1 primary antibody binds to the captured protein. Excess antibody is washed away, and bound antibody is reacted with peroxidase conjugated secondary antibody. TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of macroglobulin in the samples.
Detection Target: PAI-1
Range: 0.05 - 50 ng/ml
Sensitivity: 0.036 ng/ml
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