Rabbit Myoglobin ELISA Kit
Rabbit Myoglobin ELISA Kit is based on binding of Rabbit Myoglobin from samples to two antibodies, one immobilized on the microtiter well plates, and other conjugated to the enzyme horseradish peroxidase. After a washing step, chromogenic substrate is added and colors developed. The enzymatic reaction (color) is directly proportional to the amount of RMY present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450 nm and the concentration of RMY in samples and control is read off the standard curve.
Myoglobin is a heme protein found in both cardiac and skeletal muscle. Following myocardial necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Studies with human subjects have shown that myoglobin increases measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours. In the absence of skeletal muscle trauma or other factors associated with a noncardiac related increase in circulating myoglobin, myoglobin may used as a marker for MI. Similarly, in the absence of cardiac damage, myoglobin may be used as a marker of skeletal muscle injury.