Rabbit Fibrinogen ELISA Kit
Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis.
This rabbit fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in rabbit plasma and serum. The assay measures fibrinogen antigen in the 0.5 - 500 ng/ml range. If the unknown is thought to have high fibrinogen levels, dilutions may be made in diluent. A 1:500,000-1:1,000,000 dilution for normal rabbit plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Rabbit fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-rat fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rabbit fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.
Detection Target: Fibrinogen
Range: 0.5 - 500 ng/ml
Sensitivity: 0.065 ng/ml
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