Porcine PAI-1 ELISA Kit
PAI-1 is involved in regulating the blood fibrinolytic system. Increased plasma level of PAI-1 is involved in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Increased levels of PAI-1 tend to augment in the presence of insulin.
This porcine PAI-1 activity assay is intended for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in porcine plasma, serum and other biological fluids. The assay measures PAI-1 antigen in the 0.05 - 20 ng/ml range. If the unknown is thought to have high PAI 1 levels, dilutions may be made in plasma devoid of PAI 1 or in blocking buffer. Citrate plasma samples should be applied directly to the plate without dilution. A 1:4 dilution for EDTA plasma and 1:100 dilution for serum is suggested for best results. Strip well format. Reagents for up to 96 tests.
Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, monoclonal anti-porcine PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
Detection Target: PAI-1
Range: 0.05 - 20 ng/ml
Sensitivity: 0.046 ng/ml
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