Porcine IgM ELISA Kit
The Porcine IgM ELISA Kit is based on a sandwich ELISA. Pig IgM present in the test sample is captured by anti-pig IgM antibody that has been pre-adsorbed on the surface of microtiter wells. After sample binding, unbound proteins and molecules are washed off, and a biotinylated detection antibody is added to the wells to bind to the captured IgM. A strepavidin-conjugated horseradish peroxidase (SA-HRP) is then added to catalyze a colorimetric reaction with the chromogenic substrate TMB (3,3,5,5-tetramethylbenzidine). The colorimetric reaction produces a blue product, which turns yellow when the reaction is terminated by addition of dilute sulfuric acid. The absorbance of the yellow product at 450 nm is proportional to the amount of IgM analyte present in the sample and a four parameter standard curve can be generated. The IgM concentrations in the test samples can then be quantified by interpolating their absorbance from the standard curve generated in parallel with the samples. After factoring sample dilutions, the IgM concentrations in the original sample can finally be calculated.