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Porcine Fibrinogen Assay and ELISA Kits

Porcine Fibrinogen ELISA Kit

Catalog No.: IPFBGNKT

$395

Category: Assay and ELISA Kits

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Product Description

Porcine Fibrinogen ELISA Kit


Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis.

This porcine fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in porcine plasma and serum. The assay measures total porcine fibrinogen in the 1.56-800 ng/ml range. If the unknown is thought to have high fibrinogen levels, dilutions may be made in diluent. A 1:400,000-1:2,000,000 dilution for normal porcine plasma or up to 1:10,000 dilution for normal porcine serum is suggested for best results. Strip well format. Reagents for up to 96 tests.

Porcine fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-porcine fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of porcine fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.

Detection Target: Fibrinogen
Reactivity: Porcine
Range: 1.56 - 800 ng/ml
Sensitivity: 1.25 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
(7-14-14)

Evidence of disseminated intravascular coagulation in a porcine model following radiation exposure


GS Krigsfeld, JB Shah, JK Sanzari, L Lin, AR Kennedy - Sciences in Space Research, 2014
Thawed samples were used in the Porcine D-dimer ELISA assay (MyBioSource, San Diego, CA, USA) and the Porcine fibrinogen (Innovative Research Inc, Novi, MI, USA) ELISA assay following the vendors' protocols...

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Porcine Fibrinogen ELISA Kit

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