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Human Serum

Pooled Complement Human Serum

Catalog No.: IPLA-CSER

From $75 to $255

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$75

Category: Serum

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Product Description

Pooled Complement Human Serum


Innovative Research's Pooled Human Complement Serum is a complex mixture of serum proteins which are activated sequentially when antibodies in the blood of an immune animal interact with the corresponding antigens.

Complement proteins will quickly degrade if the serum is not prepared and stored correctly. Our complement-preserved serum is freshly-collected aseptically and kept cold, then flash frozen at -80 Celsius as soon as possible to prevent degradation of complement.

Pooled Human Complement Serum is collected at an FDA-licensed commercial donor center/facility within the United States. Each unit is tested and found negative for HBsAg, HCV, HIV-1, HIV-2, HIV-1Ag or HIV 1-NAT, ALT, and syphilis by FDA-approved methods.

This Innovative Research product is for in vitro research use only, is not to be consumed by animals or humans, and should be handled with general precaution as if capable of spreading infectious disease.

Therapeutic potential of staphylococcal superantigen-like protein 7 for complement-mediated hemolysis

Yan Li, Fiona Clow, John D. Fraser, Feng Lin
Journal of Molecular Medicine, September 2018

" ... Recombinant SSL7 and SSL7-C5a SSL7 mutant lacking C5 binding activity were overexpressed in Escherichia coli and purified following the protocol described in previous reports 16, 20. Pooled normal human serum (NHS) as the source of complement was purchased from Innovative Research (Novi, MI)."

The effect of storage temperature on the biological activity of extracellular vesicles for the complement system

Sang June Park, Hyungtaek Jeon, Seung-Min Yoo, Myung-Shin Lee
In Vitro Cellular & Developmental Biology, May 2018.

... The cells were maintained in a humidified atmosphere of 5% CO2 at 37C. Pooled complement human serum was purchased from Innovative Research, Inc. (Novi, MI) and used as normal human serum (NHS) in all experiments ...

Bystander mechanism for complement-initiated early oligodendrocyte injury in neuromyelitis optica

L Tradtrantip, X Yao, T Su, AJ Smith, AS Verkman - Acta Neuropathologica, 2017
... Complement dependent cytotoxicity Specified concentrations of AQP4-IgG (or control human IgG, Thermo Fisher Scientific, Rockford, IL) and human complement (Innovative Research, Novi, MI) were added in Hank's buffer, and cells were incubated at 37 C for specified times. ...

(5-2-2017)

Low expression of complement inhibitory protein CD59 contributes to humoral autoimmunity against astrocytes...Z Wang, W Guo, Y Liu, Y Gong, X Ding, K Shi, R Thome - Brain, Behavior, and , 2017 ... In a different set of experiments, 3 days after lentivirus injection, mice were injected in situ with 6 l IgG NMO or IgG con with 4 l human complement (hC) (Innovative Research, Novi, MI). The animals recovered normally without significant complication. ...

(11-3-15)

Antibodies produced by clonally expanded plasma cells in multiple sclerosis cerebrospinal fluid cause demyelination of spinal cord explants


K Blauth, J Soltys, A Matschulat, CR Reiter, A Ritchie - Acta Neuropathologica, 2015
... rAbs derived from MS patients and neuroinflammatory controls were applied to the media for 72 h at concentrations of 50 g/mL in the presence and absence of 10 % human complement (hComp) (pooled nor- mal human complement serum; Innovative Research, Novi, MI, USA ...

(5-5-15)

Compstatin analog Cp40 inhibits complement dysregulation in vitro in C3 glomerulopathy


Y Zhang, D Shao, D Ricklin, BM Hilkin, CM Nester - Immunobiology, 2015
...previously (Zhang et al., 2014). Whole blood was used as a source of genomic DNA, serum and plasma. Pooled normal serum (PNS, complement preserved) was purchased from Innovative Research, Inc. (Novi, MI).

(9-5-14)

Potential Therapeutic Benefit of C1-Esterase Inhibitor in Neuromyelitis Optica Evaluated In Vitro and in an Experimental Rat Model


L Tradtrantip, N Asavapanumas, PW Phuan - PLOS ONE, 2014
...PBS) and incubated at room temperature for 60 min with NMO-IgG or purified IgG from NMO patient serum in live cell buffer (PBS containing 6 mM glucose and 1 mM sodium pyruvate) containing pooled normal human complement serum (Innovative Research, Novi, MI) with or...

(6-28-14)

Inhibitor (s) of the classical complement pathway in mouse serum limit the utility of mice as experimental models of neuromyelitis optica


J Ratelade, AS Verkman - Molecular Immunology, 2014
We conclude that mice have limited utility as models of NMO. 2. Materials and methods. 2.1. Reagents and antibodies. Complement/sera from mouse (CD1 and C57BL/6 strains), rat (Wistar strain), guinea pig and human were purchased from Innovative Research (Novi, MI).

(3-31-14)

Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59


H Zhang, AS Verkman - Journal of Autoimmunity, 2014
Human complement (Innovative Research, Novi, MI) and NMO-IgG were then added in Hank's buffer, and cells were incubated at 28 C for 2 h. Cytotoxicity was measured by the Alamar Blue assay (Invitrogen). 2.5. NMO-IgG binding.

(6-20-12)

Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes


A Rossi, TJ Moritz, J Ratelade... - Journal of Cell ..., 2012 - jcs.biologists.org
...g/ml NMO or control rAb, or 2 mg/ml IgG purified from NMO or control sera plus fresh or heat-inactivated human complement (20%, pooled human complement serum, Innovative Research, Novi,MI). C1q binding was measured...

(3-5-12)

Complement-dependent cytotoxicity in neuromyelitis optica requires aquaporin-4 assembly in orthogonal arrays


PW Phuan, J Ratelade, A Rossi, L Tradtrantip... - Journal of Biological ..., 2012 - ASBMB
...18-24 h. Cells were washed with Hanks BSS (Hanks balanced salt solution, without phenol red) and incubated at 23 oC or 37 C for 60 min with NMO-IgG or NMO-rAb in Hanks BSS containing 2% pooled normal human complement serum (Innovative Research, Novi, MI) in a...

(2-26-12)

Complement-dependent cytotoxicity in neuromyelitis optica requires aquaporin-4 protein assembly in orthogonal arrays


PW Phuan, J Ratelade, A Rossi, L Tradtrantip... - Journal of Biological ..., 2012 - ASBMB
Center, Philadelphia, PA) were cultured in a Minimum Essential Medium (Invitrogen) supplemented with 10% FBS, 10% horse serum, 2.5 m...with NMO-IgG or NMO-rAb in Hanks' BSS containing 2% pooled normal human complement serum (Innovative Research, Novi, MI) in a...

(11-8-11)

Ex vivo spinal cord slice model of neuromyelitis optica reveals novel immunopathogenic mechanisms


H Zhang, JL Bennett, AS Verkman - Annals of neurology, 2011 - Wiley Online Library
...In a 3-day model, NMO-IgG (NMO-rAb or control-rAb, each 10g/ml), or purified IgG (NMO-IgG serum or control-IgG serum , 300g/ml) and/or human complement (10%, pooled normal human complement serum; Innovative Research, Novi, MI) were added on day 7 to the...

(8-2011)

Robust substrate profiling method reveals striking differences in specificities of serum and lung fluid proteases


DS Watson, K Jambunathan, DS Askew... - ..., 2011 - biotechniques.com
...Biological samples were thawed on ice immediately prior to the assay. Complement preserved normal human serum was obtained from Innovative Research (Novi, MI, USA), stored at -80C until use, and used as supplied.

(5-5-11)

Histamine release associated with intravenous delivery of a fluorocarbon-based sevoflurane emulsion in canines


RA Johnson, KT Simmons, JP Fast... - Journal of ..., 2011 - Wiley Online Library
...Complement Analysis. Pooled complement-preserved human serum (Innovative Research, Novi, Michigan) was combined with components of the emulsion to test for complement activation in vitro. Complement activation was...

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Pooled Complement Human Serum

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