Pig Cardiac Tn-I ELISA Kit
The Pig Cardiac Tn-I ELISA Kit recognizes an epitope on pig cTnI that is relatively resistant to proteolysis in pig plasma, thereby improving detection capability. The assay uses two different affinity purified antibodies. One is used for solid phase immobilization (on the microtiter wells). The second is conjugated to horseradish peroxidase (HRP). The plasma sample is diluted with three volumes of plasma diluent and allowed to react simultaneously with the two antibodies, resulting in cTnI being sandwiched between the solid phase and HRP-conjugated antibodies. After one hour incubation at room temperature on a plate shaker, the wells are washed with wash solution to remove unbound HRP-conjugated antibodies. A solution of tetramethylbenzidine (TMB), an HRP substrate, is then added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped by addition of 1N HCl, changing the color to yellow. The concentration of cTnI is proportional to the absorbance at 450 nm.