Mouse Vitronectin Total Antigen Assay
Vitronectin is an abundant plasma glycoprotein that helps regulate coagulation, fibrinolysis, complement activation, and cell adhesion. Vitronectin binds to glycosaminoglycans, collagen, plasminogen and urokinase receptors. It also may control the clearance of vascular thrombi by binding and stabilizing PAI-1. In binding PAI-1, it extends the lifetime of active PAI-1. Vitronectin may also be involved in the regulation of bone metabolism.
This mouse vitronectin total antigen assay is for the quantitative determination of total vitronectin antigen in biological fluids. The assay measures total mouse vitronectin antigen in the 0.1 - 100ng/ml range. Samples with mouse vitronectin levels above 100ng/ml should be diluted in blocking buffer before use. If the unknown is thought to have high vitronectin levels, dilutions may be made in blocking buffer. A 1:10,000-1:40,000 dilution for normal mouse plasma or serum is suggested for best results. Samples of mouse urine, cell culture media, or tissue extracts may be applied directly to the plate.
Mouse vitronectin will bind to the affinity purified capture antibody coated on the microtiter plate. Free and ligand bound enzyme will react with the antibody on the plate. After appropriate washing steps, biotinylated anti-mouse vitronectin primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse vitronectin. The amount of color development is directly proportional to the concentration of total vitronectin in the sample.
Detection Target: Vitronectin
Range: 0.1 - 100 ng/ml
Sensitivity: 0.013 ng/ml
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