Mouse uPA Assay and ELISA Kits

Mouse uPA ELISA Kit

Catalog No.: IMUPAKT


Category: Assay and ELISA Kits

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Product Description

Mouse uPA ELISA Kit

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration.

Mouse uPA activity assay is intended for the quantitative determination of active plasminogen activator in mouse plasma. The assay measures active uPA in the 0.025 - 10 ng/ml range. If the unknown is thought to have high uPA levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.

Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, anti-mouse uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.

Detection Target: Urokinase
Reactivity: Mouse
Range: 0.025 - 10 ng/ml
Sensitivity: 0.016 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Suppression of plasminogen activator inhibitor-1 by RNA interference attenuates pulmonary fibrosis

T Senoo, N Hattori, T Tanimoto, M Furonaka... - Thorax, 2010 - thorax.bmj.com
...The levels of PAI-1 and the activity of functionally active uPA in BAL fluid were measured using a total murine PAI-1 antigen assay kit (Innovative Research, Novi, Michigan, USA) and a mouse uPA activity assay kit (Innovative Research), respectively, following the...


A physiological function of inflammation-associated SerpinB2 is regulation of adaptive immunity

WA Schroder, TTT Le, L Major, S Street... - The Journal of ..., 2010 - Am Assoc Immnol
...Supernatants were concentrated 10-fold using Microcon centrifugal filter unit with a 3000 mw cutoff (Millipore, North Ryde, New South Wales, Australia), and uPA activity was measured using the Mouse uPA activity assay kit (Innovative Research, Novi, MI).


Toll-like receptor 9 signaling is critical for early experimental deep vein thrombosis resolution

PK Henke, M Mitsuya, CE Luke... - ... , and vascular biology, 2011 - Am Heart Assoc
...underwent general anesthesia with isoflorane/O 2 . All animal studies were done with University of Michigan Animal Use...thrombus homogenate was assessed for urokinase plasminogen activator and plasminogen activator inhibitor-1 by ELISA (Innovative Research, Novi, Mich).

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Mouse uPA ELISA Kit

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