Mouse uPA Assay and ELISA Kits

Mouse uPA ELISA Kit

Catalog No.: IMUPAKT


Category: Assay and ELISA Kits

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Product Description

Mouse uPA ELISA Kit

Mouse uPA ELISA Kit is for the sensitive measurement of functionally active mouse uPA is easily performed with this 96 well strip format ELISA kit. Active uPA will bind to functionally active PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate.

Functionally active uPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, monoclonal mouse anti-murine uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.

Suppression of plasminogen activator inhibitor-1 by RNA interference attenuates pulmonary fibrosis

T Senoo, N Hattori, T Tanimoto, M Furonaka... - Thorax, 2010 - thorax.bmj.com
...The levels of PAI-1 and the activity of functionally active uPA in BAL fluid were measured using a total murine PAI-1 antigen assay kit (Innovative Research, Novi, Michigan, USA) and a mouse uPA activity assay kit (Innovative Research), respectively, following the...


A physiological function of inflammation-associated SerpinB2 is regulation of adaptive immunity

WA Schroder, TTT Le, L Major, S Street... - The Journal of ..., 2010 - Am Assoc Immnol
...Supernatants were concentrated 10-fold using Microcon centrifugal filter unit with a 3000 mw cutoff (Millipore, North Ryde, New South Wales, Australia), and uPA activity was measured using the Mouse uPA activity assay kit (Innovative Research, Novi, MI).


Toll-like receptor 9 signaling is critical for early experimental deep vein thrombosis resolution

PK Henke, M Mitsuya, CE Luke... - ... , and vascular biology, 2011 - Am Heart Assoc
...underwent general anesthesia with isoflorane/O 2 . All animal studies were done with University of Michigan Animal Use...thrombus homogenate was assessed for urokinase plasminogen activator and plasminogen activator inhibitor-1 by ELISA (Innovative Research, Novi, Mich).

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Mouse uPA ELISA Kit

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